International Journal of Drug Delivery Technology
Volume 15, Issue 1

Formulation and Antibacterial Test of Moringa oleifera Seed Extract Gel Preparations on Staphylococcus aureus Bacteria

Jessica Delicia Allo1*, Prisca Deviani Pakan2, I Made Buddy Setiawan3, Iswaningsih4 

1Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Indonesia 2Department of Microbiology, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Indonesia 3Department of Surgery, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Indonesia 4Department of Public Health and Community Medicine, Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Indonesia 

Received: 25th Jan, 2025; Revised: 19th Feb, 2025; Accepted: 12th Mar, 2025; Available Online: 25th Mar, 2025

ABSTRACT

Background: Human skin can occur infection by viruses, bacteria, fungi, or parasites. One of the pathogenic bacteria that often infect the skin is Staphylococcus aureus. The incidence of bacterial infections on the skin continues to increase and pathogenic bacteria S. aureus easily experience antibiotic resistance. The development of herbal treatments from plants with antibacterial potential can be one of the alternative treatment options. One of the plants often used for alternative traditional medicine is moringa seeds (Moringa oleifera).

Purpose: Knowing and analyzing the gel formulation of moringa seed extract (Moringa oleifera), which has optimal physical characteristics and antibacterial activity against S. aureus.

Methods: The type of research conducted was true experimental with a post-test-only control group design. In this study, there was a treatment group that was given moringa seed extract gel with a concentration of 5%, 10%, 20%, 40%, and 80%, a positive control group of clindamycin gel, and a negative control group of sterile aquadest with S. aureus as test bacteria. The method of antibacterial testing uses the disc diffusion method. The results of the study were analyzed using the Kruskal-Wallis statistical test.

Results: All formulations of gel preparations have optimal physical characteristics. The diameter of moringa seed extract gel inhibition zone against S. aureus bacteria at concentrations of 5%, 10%, and 20% have a weak antibacterial potential. In comparison, a concentration of 40% (5.05 mm) is categorized as medium potential, and a concentration of 80% (11.70 mm) is classified as having strong potential. The analysis results found significant differences between treatment groups with a significance value of p < 0.05.

Conclusion: Formula 4 and 5 are optimal formulations of moringa seed extract gel preparations because they have optimal physical characteristics and antibacterial activity against S. aureus bacteria.

Keywords: Moringa oleifera, Staphylococcus aureus, Gel, Antibacterial

How to cite this article: Jessica Delicia Allo, Prisca Deviani Pakan, I Made Buddy Setiawan, Iswaningsih. Formulation and Antibacterial Test of Moringa oleifera Seed Extract Gel Preparations on Staphylococcus aureus Bacteria. International Journal of Drug Delivery Technology. 2025;15(1):86-90. doi: 10.25258/ijddt.15.1.11

 REFERENCES

  1. Menaldi, SLSW. Ilmu Penyakit Kulit dan Kelamin. Edn 7, Fakultas Kedokteran Universitas Indonesia, Jakarta, 2015, 71.
  2. Xue Y, Zhou J, Xu BN, Li Y, Bao W, Cheng XL, et al. Global Burden of Bacterial Skin Diseases: A Systematic Analysis Combined With Sociodemographic Index, 1990–2019. Front Med (Lausanne). 2022 Apr 25;9.
  3. Fathan H. Pola Resistensi Bakteri Terhadap Antibiotik Pada Akne Vulgaris Sedang Dan Berat di Departemen Ilmu Kesehatan Kulit Dan Kelamin Rumah Sakit Dr. Cipto Mangunkusumo Jakarta. 2006.
  4. Bessa GR, Machado DC, Weber MB, D’Azevedo PA, Quinto VP, Lipnharski C, et al. Staphylococcus aureus resistance to topical antimicrobials in atopic dermatitis. An Bras Dermatol. 2016 Sep 1;91(5):604–10.
  5. Yusran A, Wardana UA. Inhibition test of moringa seeds extract against the growth of Staphylococcus aureus. Makassar Dental Journal. 2020 Nov 28;9(3):202–4.
  6. Rosida, Sidiq HBHF, Apriliyanti IP. Evaluation of Physical Properties and Irritation Test of Gel Banana Peel Extract (Musa acumina Colla). Journal of Current Pharmaceutical Sciences 2018;2(1):131–135.
  7. Dewi AB, Wahyu PY. Uji Aktivitas Antibakteri Sediaan Gel Ekstrak Daun Kelor (Moringa Oleifera Lamk.) Terhadap Bakteri Staphylococcus Aureus ATCC 25923. Naskah Publikasi Sarjana Farmasi Januari. 2020.
  8. Ginarana A, Warganegara E, Oktafany. Uji Aktivitas Antibakteri Formulasi Gel Ekstrak Daun Kelor (Moringa oleifera) terhadap Staphylococcus aureus. 2020 Dec.
  9. Senduk TW, Montolalu LADY, Dotulong V. Rendemen Ekstrak Air Rebusan Daun Tua Mangrove Sonneratia alba. Jurnal Perikanan dan Kelautan Tropis [Internet]. 2020;11. Available from: https://ejournal.unsrat.ac.id/index.php/JPKT/index
  10. Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. Vol. 26, International Journal of Antimicrobial Agents. Elsevier; 2005. p. 343–56.
  11. Othman L, Sleiman A, Abdel-Massih RM. Antimicrobial activity of polyphenols and alkaloids in middle eastern plants. Vol. 10, Frontiers in Microbiology. Frontiers Media S.A.; 2019.
  12. Kaczmarek B. Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials-A minireview. Vol. 13, Materials. MDPI AG; 2020.
  13. Tagousop CN, Tamokou J de D, Kengne IC, Ngnokam D, Voutquenne-Nazabadioko L. Antimicrobial activities of saponins from Melanthera elliptica and their synergistic effects with antibiotics against pathogenic phenotypes. Chem Cent J. 2018 Sep 20;12(1).
  14. Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic Assay. 1st ed. Vol. 22. Applied Microbiology; 1971. p. 659–665.
  15. Hesti WA, Pujiyanto S, Suprihadi A. Uji Aktivitas Antibakteri Ekstrak Biji Kelor (Moringa oleifera L.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus dan Bakteri Escherichia coli. Vol. 2. 2019.
  16. Nailufar NP, Murrukmihadi M, Suprapto. Pengaruh Variasi Gelling Agent Carbomer 934 Dalam Sediaan Gel Ekstrak Etanolik Bunga Kembang Sepatu (Hibiscus Rosa-Sinensis L.) Terhadap Sifat Fisik Gel Dan Aktivitas Antibakteri Staphylococcus Aureus. Fakultas Farmasi Universitas Muhammadiyah Surakarta, Fakultas Farmasi Universitas Gajah Mada. 2013.
  17. Murphy PB, Bistas KG, le JK. Clindamycin. StatPearls Publishing; 2022 [cited 2022 Oct 10]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK519574/