International Journal of Drug Delivery Technology
Volume 15, Issue 1

Analytical Method Development and Validation of Remdesivir and Griseofulvin in API and its Dosage Form by RP-HPLC

Rakam Gopi Krishna1*, Kadagoni Pravalika2, Ramesh Konda2, V A N V Harita1, G Haritha2 

1Department of Pharmaceutical Chemistry, Marri Laxman Reddy Institute of Pharmacy, Dundigal, Hyderabad, Telangana, India-500043

2Department of Pharmaceutical Analysis, Marri Laxman Reddy Institute of Pharmacy, Dundigal, Hyderabad, Telangana, India-500043

Received: 4th Nov, 2024; Revised: 23rd Dec, 2024; Accepted: 19th Feb, 2025; Available Online: 25th Mar, 2025 

ABSTRACT

Background: The suggested approach is appropriate for the quantitative measurement of Remdesivir and Griseofulvin in a variety of pharmaceutical dosage forms, according to experimental findings. The technique offers excellent repeatability, linearity, and sensitivity. Remdesivir and Griseofulvin were estimated using RP-HPLC.

Objective: The objective was to accomplish method development and validation of Remdesivir and Griseofulvin.

Materials and Methods: The mobile phase was adjusted and consisted of acetonitrile and phosphate buffer mixed in a 70:30% v/v ratio. 4.6 was the pH of the phosphate buffer. An X Terra Symmetry C18 (4.6 x 250mm, 5µm) column is used as the stationary phase. For the detection, a UV detector tuned to 221 nm was employed. Chromatography of the solutions was performed at a constant flow rate of 1.0 ml/min. Many trials have been carried out to obtain an optimized chromatogram.

Results: Remdesivir and Griseofulvin were reported to have linearity ranges of 25-125 µg/ml. There was no increase in the linear regression coefficient above 0.999. The accuracy and precision of the procedure are indicated by the percentage RSD readings being less than 2%. The percentage recovery for Griseofulvin and Remdesivir ranges from 97 to 100%. It was discovered that LOD and LOQ were inside the range.

Conclusion: The suggested technique for figuring out how much Remdesivir and Griseofulvin are in a formulation is exact, easy to use, and accurate. A high recovery rate indicates that the formulation's excipients are not interfering with the process. Therefore, the technique may be helpful for routinely checking the quality of these medications.

Key words: Symmetry C18, Remdesivir, Griseofulvin, RP-HPLC.

How to cite this article: Rakam Gopi Krishna, Kadagoni Pravalika, Ramesh Konda, V A N V Harita, G. Haritha. Analytical Method Development and Validation of Remdesivir and Griseofulvin in API and its Dosage Form by RP-HPLC. International Journal of Drug Delivery Technology. 2025;15(1):139-45. doi: 10.25258/ijddt.15.1.19

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