International Journal of Drug Delivery Technology
Volume 15, Issue 2

Estimation of Added Urea in the Food Products and Marketed Formulation using 1,2-Napthoquinone-4-Sulphonate Reagent by UV- Visible Spectrophotometer 

K Bhavya Sri1*, T Bhagyasri1, Dhilli Rao Gorja2, C A Sri Ranjani1, P Sandhya1

1Department of pharmaceutical analysis, RBVRR women’s college of pharmacy, Hyderabad-500027, India

2MLR Institute of Technology, Laxman Reddy Avenue, Dundigal, Hyderabad, Telangana 500043, India 

Received: 22nd Oct, 2024; Revised: 8th April, 2025; Accepted: 2nd May, 2025; Available Online: 25th Jun, 2025 

ABSTRACT

Milk naturally contains urea, which makes up a large portion of the amount of non-protein nitrogen found in the milk. The amount of urea in milk varies throughout herd members. Following that, urea and the Folin (NQS) solution mix to form a vibrant complex that is visible as an absorbed range of 454 nm.

Following optimization, the limit of detection and quantification limitation were found to be the optimal experimental variables, at 0.00280 and 0.00848 µg/ml, respectively. The calibration plot for urea shows a straight line in a concentration that ranges from 0.1–10 µg/mL. The coefficient of the determination was R2 = 0.9999.

Keywords: Urea, 1,2-napthoquinone-4-sulphonate, UV Spectrophotometer.

How to cite this article: K Bhavya Sri, T Bhagyasri, Dhilli Rao Gorja, C A Sri Ranjani, P Sandhya. Estimation of added Urea in the Food Products and Marketed Formulation using 1,2-Napthoquinone-4-Sulphonate Reagent by UV- Visible Spectrophotometer. International Journal of Drug Delivery Technology. 2025;15(2): 600-04. doi:10.25258/ijddt.15.2.29

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