International Journal of Drug Delivery Technology
Volume 15, Issue 2

Simple, Rapid, Economic and Stability Indicating Method for Propranolol Hydrochloride and Flunarizine Tablets by RP-HPLC

Mashru C1, Somaiya C2, Joshi K3, Dhalani J1*

1School of Science, RK University, Rajkot-360020, Gujarat, India

2Department of Chemical Science, Parul Institute of Applied Sciences, Parul University, Vadodara-391760, Gujarat, India

3Department of Chemistry, D K V Arts and Science College, Pandit Nehru Marg, Jamnagar-361008, Gujarat, India 

Received: 13th Mar, 2025; Revised: 2nd May, 2025; Accepted: 5th Jun, 2025; Available Online: 25th Jun, 2025 

ABSTRACT

Simple, sensitive, precise, accurate, economic, and stability demonstrating high performance liquid chromatography method created and confirmed for the simultaneous evaluation of propranolol hydrochloride and flunarizine in drug substance and combined dosage form. The optimum separation was accomplished on symmetry ODS (75 x 4.6) mm, 5µm stationary phase. The mobile phase consists 550 ml of phosphate buffer pH 2.5 and 450 ml of acetonitrile at isocratic rate of 1.0 ml minute-1. Method was validated at 240 nm and 10µl injection volume.  Retention time for propranolol hydrochloride and flunarizine were achieved at 0.8 and 2.4 minutes respectively. The recommended method was linear from 1µg/mL to 7.5 µg/mL for flunarizine and from 2µg/mL to 15 µg/mL for propranolol hydrochloride. The proposed method is able to detect 0.1 µg/mL of both flunarizine and propranolol hydrochloride and able to quantify 0.2 µg/mL of both the drug substance. System suitability, linearity, recovery, filter compatibility, repeatability, intermediate precision, specificity, and solution stability criteria were taken into consideration while validating the recommended method. At the benchtop, the sample solution remained stable up to 40 hours and the standard solution up to 42 hours.

Keywords: Propranolol hydrochloride, Flunarizine dihydrochloride, RP-HPLC, Validation, Force degradation.

How to cite this article: Mashru C, Somaiya C, Joshi K, Dhalani J. Simple, Rapid, Economic and Stability Indicating Method for Propranolol Hydrochloride and Flunarizine Tablets by RP-HPLC. International Journal of Drug Delivery Technology. 2025;15(2):664-70. doi: 10.25258/ijddt.15.2.37

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