International Journal of Drug Delivery Technology
Volume 15, Issue 2

Development of Novel Nanofiber Patches Loaded with Boerhavia diffusa Root Phytochemicals for Enhanced Wound Healing: A Response Surface Methodology Approach

Smita Borkar1, Prashant Kumar Dhakad1, Hemant Kumar Singh Yadav2

1School of Pharmacy, Suresh Gyanvihar University, Jaipur, Rajasthan, India

2Department of Pharmaceutical Sciences, Indira Gandhi University, Meerpur, Rewari, India 

Received: 6th Apr, 2025; Revised: 20th May, 2025; Accepted: 10th Jun, 2025; Available Online: 25th Jun, 2025

ABSTRACT

This research focuses on the development and optimization of a novel nanofiber patch loaded with Boerhavia diffusa root extract for enhanced wound healing by Response Surface Methodology (RSM) method. Boerhavia diffusa, a traditional medicinal plant, is known for its wound healing properties through stimulation of angiogenesis, collagen formation, and epithelialization. The methanolic extract was incorporated into polyvinyl alcohol (PVA) and polyethylene oxide (PEO) solutions at 8 mg/mL concentrations and electrospun into nanofiber mats under optimized conditions.

The study employed a 32 factorial design using RSM with Design Expert-7 for evaluating drug entrapment efficiency and swelling index. Characterization techniques were applied to analyze nanofiber's morphology, structural properties, thermal behavior, and crystallinity. The optimized batch (BDRNF7) demonstrated 85.12% drug entrapment efficiency, uniform thickness, and sustained drug release. Additionally, chitosan/alginate membrane dressings were synthesized through physical blending and covalent crosslinking to evaluate film uniformity, mass, pH, and drug content. BDRNF7 showed significant wound contraction, enhanced skin breaking strength, and effective wound healing compared to controls. Moisture interaction studies indicated the patch’s stability under varying humidity. Statistical analysis confirmed the model's significance (F-value: 11.88, Adjusted R²: 0.8718). In conclusion, the developed nanofiber patch exhibited potential as a bioactive wound dressing, providing effective drug delivery and accelerated wound healing. Further studies will validate its clinical application in wound care management.

Keywords: Crosslinking, Clinical application, Wound contraction, Response Surface Methodology

How to cite this article: Smita Borkar, Prashant Kumar Dhakad, Hemant Kumar Singh Yadav. Development of Novel Nanofiber Patches Loaded with Boerhavia diffusa Root Phytochemicals for Enhanced Wound Healing: A Response Surface Methodology Approach. International Journal of Drug Delivery Technology. 2025;15(2):796-803. doi: 10.25258/ijddt.15.2.55

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