International Journal of Drug Delivery Technology
Volume 15, Issue 3

Phytochemical Characterization of Methanolic Extract of Zingiber officinale using Gas Chromatography-Mass Spectrometry

Angeline Deepthi1*, N Rakesh2, Gracelin Ranjitha E1, Deepu George Mathew3, V Shaheen Shamsuddin4, Sajna Ashraf5 

1Department of Oral Medicine and Radiology, Rajas Dental College and Hospital, Kavalkinaru junction, Tirunelveli District, Tamil Nadu-627105, India

2Department Of Oral Medicine and Radiology, M.S Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India

3Department of Oral and maxillofacial pathology, Annoor Dental college and hospital, Muvatupuzha, Kerala, India

4Kinan Dental Center, Khamis Mushayat, P.O Box-3263, Zip Code-61471, Kingdom of Saudi Arabia

5Department of Oral Medicine and Diagnostic Sciences, Vision college of Dentistry and Nursing, Riyadh, Kingdom of Saudi Arabia 

Received: 27th May, 2025; Revised: 25th Jun, 2025; Accepted: 18th Jul, 2025; Available Online: 25th Sep, 2025 

ABSTRACT

Background: Oral cancer is the sixth most common cancer worldwide and among the third in the Indian Sub-continent. Zingiber officinale Roscoe, commonly known as ginger, is generally used as an accompaniment herb or a food spice. Various in vitro and in vivo studies have demonstrated that ginger is an active compound in both the prevention and treatment of prostate, breast, ovarian, and colorectal cancers. However, the effects of ginger on oral pre-malignant conditions are still not known.

Objectives: To conduct a phytochemical analysis of the methanolic extract of Zingiber officinale by Gas Chromatography-Mass Spectrometry [GC-MS]

Materials and methods:  Zingiber officinale Roscoe (100 g) was cleaned, dried, and powdered. A Soxhlet extraction was performed using 500 mL of methanol at a temperature of approximately 60°C for 6 hours to investigate various phytochemical components. The extract was then subjected to GC-MS using the Clarus 680 GC to identify the quantitative constituents

Results: Individual compounds were analysed by GC-MS. The primary potent component identified was 6- shagoal at a peak area percentage of 35.91 %, and Zingerone, a phenolic compound with 28.16% of peak area %.

Conclusions: The existence of many secondary metabolites in the preliminary and GC-MS analysis of Zingiber officinale justifies the prevalence of phytochemicals with anti-cancer, anti-inflammatory, and other properties, like anti-angiogenic, neuroprotective properties.

Keywords: Zingiber, GC-MS, Phytotherapy, Pre-Cancer, chemoprevention.

How to cite this article: Angeline Deepthi, N Rakesh, Gracelin Ranjitha E, Deepu George Mathew, V Shaheen Shamsuddin, Sajeena Ashraf. Phytochemical Characterization of Methanolic Extract of Zingiber officinale using Gas Chromatography-Mass Spectrometry: An In-vitro Study. International Journal of Drug Delivery Technology. 2025;15(3):991-95. doi: 10.25258/ijddt.15.3.12

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