International Journal of Drug Delivery Technology
Volume 15, Issue 3

Isolation and Characterization of Novel Phenolic Compounds from the Aerial Parts of Anaphalis triplinervis (Sims) C.B. Clarke

Ankush Sundriyal1, Aanchal Loshali2, Bhuwan Chandra Joshi3,5, Minky Mukhija4, Yogita Dobhal1* 

1School of Pharmaceutical Sciences and Technology, Sardar Bhagwan Singh University, Dehradun-248001, India

2Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, India

3Department of Pharmaceutical Sciences, Faculty of Technology, Sir J.C. Bose Technical, Campus, Bhimtal, Kumaun University, Nainital-263136, Uttarakhand, India

4Ch. Devi Lal College of Pharmacy, Buria Road, Bhagwangarh, Jagadhri-135003, India

5Maharaja Agrasen Himalayan Garhwal University, Pauri Garhwal-246169, Uttarakhand, India

Received: 24th Jul, 2025; Revised: 6th Aug, 2025; Accepted: 3rd Sep, 2025; Available Online: 25th Sep, 2025 

ABSTRACT

Medicinal plants are the best source of bioactive molecules and could serve as an appreciable source of many medicines. Anaphalis triplinervis (A. triplinervis), belonging to the family Asteraceae, is a perennial shrub that commonly grows in shady, moist habitats at elevations of around 2200 meters, and is generally found in India, Pakistan, and several other parts of the world.  A. triplinervis, generally known as Bugla in Hindi and triple-veined pearly everlasting in English. The aerial parts of the plant are utilized to aid many diseases as well as serve as an important ethnomedicine. A. triplinervis is frequently used in bug bites, snake bites, as an anti-inflammatory, anti-asthmatic, anti-coughing, expectorant, sedative, and in convulsions. Isolation of ethyl acetate extract from A. triplinervis was chosen for the current investigation based on thin-layer chromatography and the first phytochemical screening. The ethyl acetate extract of the aerial parts of A. triplinervis was subjected to column chromatography following successive solvent extractions to isolate individual compounds. Every fraction of 20ml has been collected, and a total of 491 fractions were taken. Two phenolic compounds were isolated and initially confirmed by spraying a dilute ferric chloride solution on TLC plate.  Further structure elucidation has been conducted by utilizing several spectrophotometric methods such as UV, IR, 1H &13C NMR, along with mass spectroscopy (MS). Based on spectral analysis, the compound was identified as Quercetin 3,7-di-O-rhamnoside, a flavonoid, along with caffeic acid, a phenolic acid.

Keywords: Anaphalis triplinervis, extraction, isolation, characterization

How to cite this article: Ankush Sundriyal, Aanchal Loshali, Bhuwan Chandra Joshi, Minky Mukhija, Yogita Dobhal. Isolation and Characterization of Novel Phenolic Compounds from the Aerial Parts of Anaphalis triplinervis (Sims) C.B. Clark. International Journal of Drug Delivery Technology. 2025;15(3):1017-21. doi: 10.25258/ijddt.15.3.16

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