International Journal of Drug Delivery Technology
Volume 15, Issue 3

Development and Validation of UV Spectroscopic Method for Simultaneous Estimation of Adapalene and Quercetin

Chaudhari Pallavi Manojkumar*, Bhotkar Priyanka Rangrao 

Department of Pharmaceutics, Dr. D.Y. Patil Pratishthan’s, Dr. D.Y. Patil College of Pharmacy, Akurdi, Affiliated to Savitribai Phule Pune University (SPPU), Pune, Maharashtra-411044, India 

Received: 28th Aug, 2025; Revised: 7th Sep, 2025; Accepted: 12th Sep, 2025; Available Online: 25th Sep, 2025 

ABSTRACT

Background: The UV Spectrophotometric method is quick, cost-effective, accurate simple, rapid and precise was developed and validated for simultaneous estimation of Adapalene and Quercetin. Adapalene having anti-acne activity, Comedolytic effect, Keratolytic effect, and Quercetin having anti-inflammatory, Antibacterial, Wound healing activity   Purpose: To develop and validate simultaneous equation method for Adapalene and Quercetin.

Materials and method: This method involved estimation using two wavelengths. Since the absorbance of Adapalene and Quercetin reaches its maximum at 320 and 370 nm, respectively, these wavelengths were used to measure the absorbance and to estimate their relative concentrations. Adapalene and Quercetin both follow Beer-Lambert's rule at concentrations between 2-10 µg/mL.Dimethyl sulfoxide (DMSO) was used as common solvent.

Result: This method was validated with respect to Linearity, accuracy, precision, Limit of Detection and quantification as per ICH norms. %RSD was found to be less than 2. Linear regression coefficient for Adapalene and Quercetin was found to be 0.9987 and 0.9983 respectively. For Adapalene, the LOD and LOQ values attained are   0.111 µg/mL and 0.33µg/mL. Also, the LOD and LOQ values attained for Quercetin are 3.40 µg/mL and 3.16 µg/mL, correspondingly. Thus, this method was found accurate, precise, and reproducible for simultaneous estimation of Adapalene and Quercetin

Keywords: Adapalene, Quercetin, UV-Spectrometry, Simultaneous estimation, Vierordt's techniqu

How to cite this article: Chaudhari Pallavi Manojkumar, Bhotkar Priyanka Rangrao. Development and Validation of UV Spectroscopic Method for Simultaneous Estimation of Adapalene and Quercetin. International Journal of Drug Delivery Technology. 2025;15(3):1090-93. doi: 10.25258/ijddt.15.3.25

REFERENCES

  1. Ahmad Nasrollahi S, Koohestani F, Naeimifar A et al. Preparation and Evaluation of Adapalene Nanostructured Lipid Carriers for Targeted Drug Delivery in Acne. Dermatologic Therapy. 2021;34(2):e14777. DOI:10.1111/dth.14777
  2. Halagali P, Wannur VI, Kumar AKA et al. Formulation and Evaluation of Quercetin Ethosomal Hydrogel for Topical Delivery System. International Journal of Pharmaceutical Investigation. 2024;14(3):749-58. DOI: 11.2.1
  3. Sahoo NG, Kakran M, Shaal LA, et al. Preparation and Characterization of Quercetin Nanocrystals. Journal of Pharmaceutical Sciences. 2011;100(6):2379-90. DOI:10.1002/jps.22446
  4. Kamal AH, El-Malla SF, Hammad SF. A Review on UV Spectrophotometric Methods for Simultaneous Multicomponent Analysis. European Journal of Pharmaceutical and Medical Research. 2016;3(2):348-60
  5. Ravindra K. Simultaneous Estimation and Statistical Evaluation of Developed Validated Methods for Combined Drugs in Marketed Formulation. Journal of Pharmaceutical Research. 2013;12(1):23-9
  6. Gupta D, Bhardwaj S, Sethi S et al. Simultaneous Spectrophotometric Determination of Drug Components From Their Dosage Formulations. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy. 2022;270:120819. DOI:10.1016/j.saa.2021.120819
  7. Vyas AJ, Jha SA, Patel AB. Review on Simultaneous Equation Method (Vierodt’s Method). Asian Journal of Pharmaceutical Analysis. 2022;12(2):149-56. DOI:10.52711/2231-5671.2022.00026
  8. Korany MA, Wahbi AM, Mandour S, Elsayed MA. Determination of Certain Drugs in Multicomponent Formulations by First Derivative Ultraviolet Spectrophotometry. Analytical Letters. 1985;18(1):21-34. DOI:10.1080/00032718508066922
  9. Stenlake JB. Practical pharmaceutical chemistry. London: Athlone Press; 1975
  10. Rao CR, Shinozakt N. Precision of Individual Estimators in Simultaneous Estimation of Parameters. Biometrika. 1978;65(1):23-30. DOI:10.1093/biomet/65.1.23
  11. Sen AK, Sen DB, Maheshwari RA, Balaraman R, Seth A. Simultaneous Estimation of Aliskiren Hemifumarate and Hydrochlorothiazide in Combined Tablet Formulation by Simultaneous Equation, Absorbance Ratio and First Derivative Spectroscopic Methods. Journal of Applied Pharmaceutical Science. 2016;6(7):164-70. DOI:10.7324/JAPS.2016.60724
  12. Dhartarkar PG, Kalamkar RV, Suprit D. Development and Validation of UV Spectrophotometric Method for Estimation of Dexibuprofen in Bulk and Dosage Form. Scholars Res Library Pharmaceutical Chem. 2011;3(4):361-6
  13. Singh U, Baldi A. Simultaneous Estimation of Quercetin and Silymarin: Method Development and Validation. International Journal of Pharmaceutical and Biological Archives. 2013;4(3):527-31
  14. Chaudhari S, Mannan A, Daswadkar S. Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Acyclovir and Silymarin in Niosome Formulation. Scholar Research Library. 2016;8(5):128-33
  15. Khanage SG, Mohite PB, Jadhav S. Development and Validation of UV-Visible Spectrophotometric Method for Simultaneous Determination of Eperisone and Paracetamol in Solid Dosage Form. Advanced Pharmaceutical Bulletin. 2013;3(2):447-51. DOI:10.5681/apb.2013.073
  16. Gholse YN, Chaple DR, Kasliwal RH. Development and Validation of Novel Analytical Simultaneous Estimation-Based UV Spectrophotometric Method for Doxycycline and Levofloxacin Determination. Bio Interface Research in Applied Chemistry. 2022;12(4):5458. DOI:10.33263/BRIAC12454585478
  17. Celia C, Di Marzio L, Locatelli M et al. Current Trends in Simultaneous Determination of Co-administered Drugs. Separations. 2020;7(2):29. DOI:10.3390/separations7020029
  18. Chaudhary J, Jain A, Saini V. Analytical Method Development and Validation for the Simultaneous Estimation of Alprazolam and Propranolol in Their Combined Dosage Form. International Journal of Drug Delivery. 2012;4(3):310-5