International Journal of Drug Delivery Technology
Volume 15, Issue 3

Isolation, Identification and Screening of the In vitro Cytotoxicity of the Phytochemicals from Duranta erecta (Verbenaceae) 

Wagh A S1*, Butle S R2, Jadhav P B3, Bhokare S G1 

1Srinath College of Pharmacy, Aurangabad, Maharashtra, India

2School of Pharmacy, S R T M University, Nanded, Maharashtra, India

3School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, India

Received: 18th Jun, 2025; Revised: 25th Jul, 2025; Accepted: 23rd Aug, 2025; Available Online: 25th Sep, 2025

ABSTRACT

The main objective of the present study was to investigate cytotoxic activity of the phytoconstituent of Duranta erecta L. against the HL-60 cell line using SRB assay. Column chromatographic method & Preparative Thin Layer Chromatography was used for the separation of phytochemicals from chloroform extract of the stem of Duranta erecta. Three isolated compounds were identified as well as confirmed by using different analytical techniques like UV, FTIR, 1H & 13C NMR and MS. In vitro cytotoxic activity of these compounds was screened using SRB assay against Leukemia cancer cell lines in humans (HL-60). The compounds Ursolic acid, Pyrocatechol and Kaempferol were separated and confirmed from the chloroform extract. All extracted compounds exhibited inhibition of the HL-60 cell lines and showed IC50 values less than 165µg/ml. Moreover, Ursolic acid (IC5098.6µg/ml) was more cytotoxic than other isolated compounds when tested on the cell line model.The statistically significant difference (P<0.05) in cytotoxic activity of extract was observed relative to standard compound when examined by independent student t test. Amongst three compounds Ursolic acid anticancer activity was different by statistic (P<0.05) than Pyrocatechol as well as Kaempferol. Additionally, the compounds which were isolated from the extract of chloroform of D. erecta show dose-dependent cytotoxic activity on HL-60 cell lines. This study suggested that the Duranta erecta is a promising source of bioactive chemicals having anticancer activity which could be used in the cancer drug discovery process.

Keywords: Duranta erecta; Cytotoxic activity; Chloroform extract; SRB assay; Human leukemia cancer cell line

How to cite this article: Wagh A S, Butle S R, Jadhav P B, Bhokare S G. Isolation, Identification and Screening of the In vitro Cytotoxicity of the Phytochemicals from Duranta erecta (Verbenaceae). International Journal of Drug Delivery Technology. 2025;15(3):1212-19. doi: 10.25258/ijddt.15.3.41

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