International Journal of Drug Delivery Technology
Volume 15, Issue 3

Solubility Profiling and UV-Spectrophotometric Determination of Bioactive Peptides from Ragi 

Sawant M P*, Deokar G S

MET’s Institute of Pharmacy, Bhujbal Knowledge City, Adgaon, Nashik, Maharashtra-422003, India (Affiliated to Savitribai Phule Pune University, Pune, India

Received: 8th Apr, 2025; Revised: 4th Jul, 2025; Accepted: 14th Aug, 2025; Available Online: 25th Sep, 2025 

ABSTRACT

Objective: The primary aim was to determine the solubility of isolated bioactive peptides (BPs) from Ragi (Eleusine coracana L.) A review of the literature revealed that there is no UV-visible method to measure bioactive peptides is developed yet. Therefore, it is necessary to design and authenticate an accurate, simple, exact, durable and economical UV visible spectrophotometric method of the quantification of bioactive peptides derived from Ragi.

Method: The solubility of bioactive peptides derived from Ragi was evaluated in DMSO, methanol, and buffers at pH levels of 1.2, 4.5 (Acetate and phosphate), 6.8, 7.4 and 10, using the saturated solubility determination technique. A standard solution was prepared using the selected solvent, and the maximum absorbance wavelength (λmax) was identified. Subsequently, a calibration curve was constructed based on absorbance measurements. The method's analytical performance was assessed by evaluating parameters including linearity, range, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness, in accordance with the International Conference on Harmonisation (ICH) Q2 (R2) guidelines.

Result: Bioactive peptides isolated from Eleusine coracana (ragi) exhibited highest solubility in phosphate buffer at pH 7.4, followed by buffers at pH 6.8, pH 10, and organic solvents such as dimethyl sulfoxide (DMSO) and methanol. The peptides showed maximum absorbance at a wavelength of 379 nm. A linear response consistent with the Beer-Lambert law was observed over the concentration range of 100–1000 µg/ml, yielding a correlation coefficient (R²) of 0.993. The method demonstrated a limit of detection (LOD) of 2.89 µg/ml and a limit of quantification (LOQ) of 8.76 µg/ml. Precision and repeatability assessments were within acceptable regulatory thresholds, with relative standard deviation (RSD) values below 2%. Recovery studies indicated an accuracy range between 98.23% and 100.03%.

Conclusion: The study concluded that the isolated BPs demonstrate diverse solubility in various solvents. The established process was accurate, exact, reproducible, easy, ecologically sustainable, repeatable, and cost-effective, rendering it appropriate for quantifying BPs in pharmaceutical dosage forms.

Keywords: Bioactive peptides isolated from Ragi, UV visible spectrophotometry, Method development and Validation, Eleusine coracana L

How to cite this article: Sawant M P, Deokar G S. Solubility Profiling and UV-Spectrophotometric Determination of Bioactive Peptides from Ragi. International Journal of Drug Delivery Technology. 2025;15(3):1265-71. doi: 10.25258/ijddt.15.3.47

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