Structure-Based Virtual Screening in Oncology: Repurposing FDA-Approved Drugs for Breast Cancer
Ashwini Dighe, Ganesh Dama*, Sumit Joshi, Asmita Gaikwad
SGMSPM’s Sharadchandra Pawar College of Pharmacy, Otur, Tal.- Junnar, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India
Received: 9th Jun, 2025; Revised: 11th Jul, 2025; Accepted: 28th Jul, 2025; Available Online: 25th Sep, 2025
ABSTRACT
Structure-based virtual screening (SBVS) has been recently established as a significant and fruitful computational method in drug repurposing, where drugs already approved by the FDA are screened for their potential therapeutic effects against breast cancer. This review lays down the principles of SBVS while broaching important computational programs such as molecular docking software, molecular dynamic simulation, and AI-driven predictive models. Databases of drug repositioning such as DrugBank, PubChem, and ChEMBL provide comprehensive datasets mandatory for virtual screening. The past few years have seen SBVS emerge as a tool for drug repurposing with exciting new candidates. Some of these are metformin, statins, NSAIDs, beta-blockers, and antidepressants, with anti-cancer activities reported through a plethora of mechanisms. However, accuracy limits, experimental validation, and regulatory hurdles impede clinical translation. The way forward envisions approaches integrating AI, multi-target drug strategies, and personalized medicine to enhance SBVS's suitability and reliability in oncology. Utilizing computational methods and an inter-disciplinary dynamic, drug repurposing with SBVS is anticipated to get very far and create a viable toolkit for breast cancer therapeutic discovery.
Keywords: Structure-based virtual screening, drug repurposing, breast cancer, docking, AI in drug discovery, molecular dynamics simulation, repositioning databases, computationally assisted drug design, targeted therapies, personalized medicine.
How to cite this article: Ashwini Dighe, Ganesh Dama, Sumit Joshi, Asmita Gaikwad. Structure-Based Virtual Screening in Oncology: Repurposing FDA-Approved Drugs for Breast Cancer. International Journal of Drug Delivery Technology. 2025;15(3):1395-99. doi: 10.25258/ijddt.15.3.62
REFERENCES
- Kashyap D, Pal D, Sharma R, Garg VK, Goel N, Koundal D, Zaguia A, Koundal S, Belay A. [Retracted] Global Increase in Breast Cancer Incidence: Risk Factors and Preventive Measures. BioMed research international. 2022;2022(1):9605439. https://doi.org/10.1155/2022/9605439
- Wang Y, Minden A. Current molecular combination therapies used for the treatment of breast cancer. International journal of molecular sciences. 2022 Sep 20;23(19):11046. https://doi.org/10.3390/ijms231911046
- Pinzi L, Bisi N, Rastelli G. How drug repurposing can advance drug discovery: challenges and opportunities. Frontiers in Drug Discovery. 2024 Aug 20;4:1460100. https://doi.org/10.3389/fddsv.2024.1460100
- Jan N, Sofi S, Qayoom H, Shabir A, Haq BU, Macha MA, Almilaibary A, Mir MA. Metronomic chemotherapy and drug repurposing: A paradigm shift in oncology. Heliyon. 2024 Feb 15;10(3). https://doi.org/10.1016/j.heliyon.2024.e24670
- Rao MR, Ghadge IS, Kulkarni SP, Asthana T. Computational techniques for drug repurposing: a paradigm shift in drug discovery. Current Drug Therapy. 2023 Aug 1;18(4):271-84. https://doi.org/10.2174/1574885518666230207143523
- Weth FR, Hoggarth GB, Weth AF, Paterson E, White MP, Tan ST, Peng L, Gray C. Unlocking hidden potential: advancements, approaches, and obstacles in repurposing drugs for cancer therapy. British Journal of Cancer. 2024 Mar 23;130(5):703-15. https://doi.org/10.1038/s41416-023-02502-9
- Wang T, Pulkkinen OI, Aittokallio T. Target-specific compound selectivity for multi-target drug discovery and repurposing. Frontiers in pharmacology. 2022 Sep 23;13:1003480. https://doi.org/10.3389/fphar.2022.1003480
- Al-Taie Z, Hannink M, Mitchem J, Papageorgiou C, Shyu CR. Drug repositioning and subgroup discovery for precision medicine implementation in triple negative breast cancer. Cancers. 2021 Dec 14;13(24):6278. https://doi.org/10.3390/cancers13246278
- Sohraby F, Aryapour H. Rational drug repurposing for cancer by inclusion of the unbiased molecular dynamics simulation in the structure-based virtual screening approach: Challenges and breakthroughs. InSeminars in Cancer Biology 2021 Jan 1 (Vol. 68, pp. 249-257). Academic Press. https://doi.org/10.1016/j.semcancer.2020.04.007
- Prajapati V, Verma M, Kumar GS, Patel J. High-Throughput Preclinical Models and Pharmacoproteomics. InPharmacoproteomics: Recent Trends and Applications 2024 Sep 1 (pp. 429-468). Cham: Springer Nature Switzerland. https://doi.org/10.1007/978-3-031-64021-6_18
- Aziz M, Ejaz SA, Zargar S, Akhtar N, Aborode AT, A. Wani T, Batiha GE, Siddique F, Alqarni M, Akintola AA. Deep learning and structure-based virtual screening for drug discovery against NEK7: a novel target for the treatment of cancer. Molecules. 2022 Jun 25;27(13):4098. https://doi.org/10.3390/molecules27134098
- Garg P, Singhal G, Kulkarni P, Horne D, Salgia R, Singhal SS. Artificial Intelligence–Driven Computational Approaches in the Development of Anticancer Drugs. Cancers. 2024 Nov 20;16(22):3884. https://doi.org/10.3390/cancers16223884
- Jakhmola-Mani R, Sharma V, Singh S, Allen T, Dogra N, Katare DP. Drug Repurposing and Molecular Insights in the Fight Against Breast Cancer. Biomedical and Pharmacology Journal. 2024 Jun 4;17(2). https://dx.doi.org/10.13005/bpj/2907
- Kandasamy T, Sarkar S, Ghosh SS. Harnessing Drug Repurposing to Combat Breast Cancer by Targeting Altered Metabolism and Epithelial-to-Mesenchymal Transition Pathways. ACS Pharmacology & Translational Science. 2024 Oct 31;7(12):3780-94. https://doi.org/10.1021/acsptsci.4c00545
- Yousif FA, Alzain AA, Alraih AM, Ibraheem W. Repurposing of approved drugs for targeting CDK4/6 and aromatase protein using molecular docking and molecular dynamics studies. Plos one. 2023 Sep 8;18(9):e0291256. https://doi.org/10.1371/journal.pone.0291256
- Dhas Y, Biswas N, MR D, Jones LD, Ashili S. Repurposing metabolic regulators: Antidiabetic drugs as anticancer agents. Molecular Biomedicine. 2024 Sep 28;5(1):40. https://doi.org/10.1186/s43556-024-00204-z
- To KK, Cho WC. Drug Repurposing to Circumvent Immune Checkpoint Inhibitor Resistance in Cancer Immunotherapy. Pharmaceutics. 2023 Aug 21;15(8):2166. https://doi.org/10.3390/pharmaceutics15082166
- Xu X, Liu A, Liu S, Ma Y, Zhang X, Zhang M, Zhao J, Sun S, Sun X. Application of molecular dynamics simulation in self-assembled cancer nanomedicine. Biomaterials Research. 2023 May 4;27(1):39. https://doi.org/10.1186/s40824-023-00386-7
- Pun FW, Ozerov IV, Zhavoronkov A. AI-powered therapeutic target discovery. Trends in pharmacological sciences. 2023 Sep 1. https://doi.org/10.1016/j.tips.2023.06.010
- Amengual-Cladera E, Morla-Barcelo PM, Morán-Costoya A, Sastre-Serra J, Pons DG, Valle A, Roca P, Nadal-Serrano M. Metformin: From Diabetes to Cancer—Unveiling Molecular Mechanisms and Therapeutic Strategies. Biology. 2024 Apr 27;13(5):302. https://doi.org/10.3390/biology13050302
- Maja M, Tyteca D. Alteration of cholesterol distribution at the plasma membrane of cancer cells: From evidence to pathophysiological implication and promising therapy strategy. Frontiers in physiology. 2022 Nov 9;13:999883. https://doi.org/10.3389/fphys.2022.999883
- Kapoor G, Prakash S, Jaiswal V, Singh AK. Chronic Inflammation and Cancer: Key Pathways and Targeted Therapies. Cancer Investigation. 2024 Dec 7:1-23. https://doi.org/10.1080/07357907.2024.2437614
- Liao M, Zhang J, Wang G, Wang L, Liu J, Ouyang L, Liu B. Small-molecule drug discovery in triple negative breast cancer: current situation and future directions. Journal of medicinal chemistry. 2021 Mar 2;64(5):2382-418. https://doi.org/10.1021/acs.jmedchem.0c01180
- Mishra AS, Vasanthan M, Malliappan SP. Drug Repurposing: A Leading Strategy for New Threats and Targets. ACS Pharmacology & Translational Science. 2024 Apr 1;7(4):915-32. https://doi.org/10.1021/acsptsci.3c00361
- Pandey AK, Verma S. Computational Approaches for Structure-Assisted Drug Discovery and Repurposing. InUnraveling New Frontiers and Advances in Bioinformatics 2024 Sep 22 (pp. 163-192). Singapore: Springer Nature Singapore. https://doi.org/10.1007/978-981-97-7123-3_9