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In current study, an attempt has been taken for formulation and evaluation acetone extract of Plumbago indica for antiacne activity. The gel containing acetone extract of Plumbago indica was prepared by adding gel-forming material in sterile distilled water while the mixture was stirred and allowed to stand to hydrate. Extract was added in PEG 400 and polyethylene glycol mixture. Methyl paraben and propyl paraben were added as the preservative and triethanolamine was added as the neutralizer. The anti-acne activity was investigated against two causative bacteria, i.e., Propionibacterium acnes and Staphylococcus epidermidis and yeast (Malassezia furfur) by the well diffusion method. The result showed that gel passes all test of evaluation and was found to be active against acne causing microorganisms. Plumbago indica extract based gel has potential activity against acne-causing microorganism.