Purification of levanase from Enterobacter aerogenes and using it as a prebiotic and antibacterial agent.

Sahira Nsayef Muslim, Sawsan Mohammed Kareem

Abstract


A novel strain of Enterobacter aerogenes isolated from rhizosphere soil has an ability to produce intracellular levanase when grown on basal medium containing the levan as substrate. Levanase was purified to homogeneity with a recovery yield of 57.85% and 22.5 fold of purification by using ammonium sulfate at 40% saturation followed by ion exchange chromatography on Sepharose CL-6B column and gel filtration chromatography on Sephadex G-100 column. The purified levanase has a potential prebiotic property of enhancing growth of lactic acid bacteria while apparently inhibiting growth of pathogenic bacteria. Typically, prebiotic properties should not only reduce growth of pathogens but must also promote growth of desirable and beneficial bacteria, such as Lactobacillus. Thus the levanase may be used in animal feed to improve digestibility and support gastrointestinal health.

Keywords


Levanase, purification, prebiotic agent

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DOI: https://doi.org/10.25258/ijddt.v9i3.12

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