Quantification of Doxycycline Hyclate in Different Pharmaceutical Samples by UV Assay

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Md Mahmudul HasaN Kazi
Md Anwarul Haque
Sm Alamgir Kobir
Mobarak Hossain

Abstract

Objective: Doxycycline hyclate is a broad spectrum antibiotic with activity against a wide range of gram-positive and
gram-negative bacteria and it is widely used as a pharmacological agent. A simple, selective, linear, precise and accurate
ultraviolet detection (UV) method has been developed and applied for the determination of doxycycline hyclate in different
pharmaceutical samples. Methods: Acid-base analysis and titrimetric method were utilized to determine the value of pH
and moisture content of purchased pharmaceutical samples. A mixture of methanol and hydrochloric acid (0.01N
Methanolic HCl) was used to determine the biochemical properties of doxycycline hyclate. UV detector set at 349 nm was
used to monitor the effluent. The purified water was used as solvent. Results: In 1% aqueous solution of doxycycline, three
samples (4th, 5th and 7th) showed lower pH values of 1.97, 1.98, and 1.99 respectively. Furthermore, the same samples
indicated the additional moisture contents of 2.81%, 2.85% and 2.83% respectively while considering the acceptance level
(1.4% to 2.8%). The method proved to be linear (R2=0.993), precise (RSD=0.79% for inter-day precision), accurate
(Recovery=100.59%) and selective regarding possible impurities and excipients of the samples. The doxycycline content
obtained in the sample analysis was within the range of 84.05% to 85.80%. Conclusion: The optimized and validated
method may be successfully employed to perform routine quality control analyses. Investigation of the pH, moisture
content and potency of doxycycline hyclate in different samples give a general view of local pharmacies trade and ensure
that the method applied here was validated for this kind of analysis.

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Research Article