Detection and in silico Analysis of rmpA Gene from Soilborne Pathogenic Bacteria

Laith AbdulHassan Mohamed-Jawad

Abstract


Soil serves as an ecosystem for diverse microbes that perform various roles and that range from useful organisms in biological and geological processes to dangerous transmitters of diseases. Most type of soilborne bacteria are harmful to humans and causing severe problems. Klebsiella pneumoniae consider a serious nosocomial pathogen that cause pneumonia, UTIs, wound and liver abscesses. One hundred fifty (150) soil samples isolated randomly during the period November 2014- February 2015. Bacterium Klebsiella pneumoniae was the commonest pathogen (36.70%) followed by Staphylococcus aureus (22.78%), Staphylococcus epidermidis (12.65%), Bacillus spp. (10.12%), Pseudomonas aeruginosa (5.06%), Enterobacter cloacae (5.06%), Streptococcus spp.(2.53%), Citrobacter spp.(1.26%) and Pantoea spp.(1.26%) respectively. All K. pneumoniae isolates detected and confirmed by conventional PCR using rmpA gene size 530bp. The positive isolates were sequenced and search for homology. The multiple sequence alignment showed that our bacterial gene have differences in NAD-dependent epimerase and NAD (p)- binding domain sequence at different sites. The differences in the nucleotides at gene sequence for the isolated strain which may give new approach for developing higher sensitive K. pneumoniae strains and for better understanding of the rmpA gene structure that should provide new tools for disease management.
Keywords:

Keywords


In silico, Klebsiella pneumonia, Soilborne, Phylogeny

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DOI: https://doi.org/10.25258/ijddt.v9i3.19

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