Detection of combination effect of silver nanoparticles prepared with Pimpinella anisum and some antibiotics on some pathogenic bacteria

Hussein A. R. Abbood, Salah S. Zain alabden


This article aims to detect the effect of combining silver nanoparticles prepared with the extract of the Pimpinella anisum seeds and was obtained from the local markets in the city of Kirkuk. Iraq with some antibiotics on antibiotic resistance bacteria (E.coli and Staphylococcus aureus ) were obtained from Kirkuk General Hospital and re-diagnosed based on reliable diagnostic sources. This study demonstrates that the P. anisum seeds extract reduced the silver ion through changing the solution color to reddish brown. The examination of silver nanoparticles synthesized by P. anisum the extract of seeds was carried out using X-ray and SEM (Scanning electron microscopy) and X-ray showed spherical shape particles with 44 nm in size with three diffractions which are indexed as (111), (200) and (220)and the SEM images showed clear particales. The synergistic effects of biosynthesis AgNPs at different concentrations with different standard antibiotic discs (Amoxicillin, chloramphenicol, Erythromycin, Ciprofloxacin) against multi-antibiotic resistance bacteria were also investigated. The result showed the synergistic action of AgNPs and antibiotics leading to enhance antibacterial activity.


Pimpinella anisum , silver nanoparticals , pathogenic bacteria

Full Text:




  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.