Effect of exchange of gases for tissue culture vessels to produce the meristem of Solanum tuberosum in vitro

Malek Hubaish Walli, Haider Mahmooed Jasim, Faiq Hassan Al

Abstract


The microenvironment in plant tissue culture vessels has a significant effect on the growth and development of plantlets in vitro. The Studies have indicated the gas exchange between outside and inside air can effect on microenvironment of culture vessels, therefore, the experiment was conducted to determine the effect of gas exchange on the production of the fabric tissue to micropropagation of Solanum tuberosum and the possibility of storage the fabric to a long time without drought to the culture. In the experiment, two factors were used. The first factor is the volume of gas exchange of the diameter of the filter (1 cm, 1.5 cm, 2 cm, 2.5 cm) and the second factor used four varieties of Solanum tuberosum (Eastma, Red Scarlet, Arora, Violet ).

Keywords


Tissue culture, Solanum tuberosum, Micropropagation and Gas exchange

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DOI: https://doi.org/10.25258/ijddt.v9i3.27

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