Peel-off Gel Formulation From Black Mulberries (Morus Nigra L.) Leaves Extract as a Tyrosinase Inhibitor

Arif Budiman1, Zelika Mega R, Nadiatul Khaira Y, Diah Lia Aulifa3


Hyperpigmentation is a skin pigmentation disorder caused by increasing melanin production. Tyrosinase enzyme activity plays a role in the melanin pigment synthesis in the skin using tyrosine as a substrate. An inhibitor of tyrosinase enzyme activity is the most prominent method for preventing hyperpigmentation. Morus nigra L. (MN) leaves with high flavonoids and polyphenols are the potential material to prevent hyperpigmentation by inhibiting tyrosinase enzyme activity. The aim of this study was to formulate and evaluate a peel-off gel mask of MN leaves extract (Morus nigra L.), which can inhibit tyrosinase enzyme activity. The MN leaves were extracted by a maceration method using ethanol (70%) as a solvent. The inhibitor of tyrosinase enzyme activity from MN leaves extract was determined by in-vitro study. The extract was formulated into a base of a peel-off gel mask containing variations in the concentration of polyvinyl alcohol (10, 12.5, and 15%) and gelling agents. The peel-off gels of MN leave extract were evaluated, including organoleptic, homogeneity, pH, viscosity, spreadability, and drying time. Irritation tests were performed, and the test of preference of the formulation was also assessed. The result showed that MN leaves extract has an enzyme inhibitor activity of tyrosinase with IC50 of 511.91 μg/mL. The formulation containing 1.5 % of MN leaves extract, 15 % of polyvinyl alcohol, and 0.5 % of carbomer showed the best physical stability results and safe for topical preparations. It can be concluded that the peel-off mask gel prepared from MN leaves extract is potential for preventing hyperpigmentation as well as safe to be used as a topical preparation.


Morus nigra L. leaves extract, Peel-off mask gel, Tyrosinase.

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