Drug Eluting Stent: Development and Validation of RP-HPLC Method for Simultaneous Estimation of Gatifloxacin and flurbiprofen Sodium in Eye Drops

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Pinkal Patel
Nalini Patel
Kinjal Parmar

Abstract

A simple, selective and rapid reversed phase High Performance Liquid Chromatographic (RP-HPLC) method has been
developed and validated for the simultaneous analysis Gatifloxacin and flurbiprofen sodium in eye drops. The separation
was carried out using a mobile phase consisting ACN: Buffer (pH 3.5) in the ratio of 55:45 v/v. The column used was
Phenomenex luna ODS C18 (250mm X 4.6 mm i.d., 5 μm particle size) with flow rate of 1 ml / min using UV detection
at 268 nm. The described method was linear over a concentration range of 2-12 μg/ml for both of Gatifloxacin and
flurbiprofen sodium. The retention times of Gatifloxacin and flurbiprofen sodium were found to be 3.710 min. and 6.797
min respectively. Method was validated statistically and recovery studies were carried out. The proposed method has
been applied successfully to the analysis of cited drugs either in pure form or in pharmaceutical formulations with good
accuracy and precision. The method here in described can be employed for quality control and routine analysis of drugs
in pharmaceutical formulations.

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Research Article