Development and Validation of a Simple HPLC-UV Method for The Quantification of Andrographolide In Rabbit Plasma

Syukri Y., Widarno S., Adewiyah A., Wibowo A., Martien R., Lukitaningsih E., Nugroho E.

Abstract


In this present work, a simple, rapid and accurate HPLC-UV method has been developed for the quantification of
andrographolide in rabbit plasma. The assay was performed using an XTerra® MS C18 column (150 mm X 4.6 mm, 5 μm)
with a mobile phase of methanol and water (60:40), at 0.8 mL/min flow rate and UV detection of 229 nm. Andrographolide
was extracted from a biological sample by applying acetonitrile as a precipitating and extraction solvent. The results
showed a good linearity with r = 0.9992; the accuracy reported as % diff was found to be -6.42 – 6.55 % while the recovery
was 99.09, 98.55, and 105.14% for low, medium and high spiked plasma, respectively. The precision (reproducibility)
reached 1.08–3.20 % RSD for the sample studied. The 2.87 % relative standard deviation (RSD) value for selectivity test
indicated a good selectivity of the developed method. The developed method is simple and rapid, so that it can be applied
for the quantification andrographolide in animal models during pharmacokinetics studies.

Keywords


HPLC-UV, andrographolide, validation, biological sample

Full Text:

PDF


DOI: https://doi.org/10.25258/ijddt.v7i1.8913

Refbacks

  • There are currently no refbacks.