International Journal of Drug Delivery Technology
Volume 14, Issue 1

Extraction, Isolation and Neuropharmacological Potential of Phyllanthus acidus L.

Nripen Khare, Harshal Tare, Sachin K Jain

Faculty of Pharmacy, Oriental University, Indore, Madhya Pradesh, India. 

Received: 18th December, 2023; Revised: 21st January, 2024; Accepted: 06th March, 2024; Available Online: 25th March, 2024

ABSTRACT

Because of their broad availability, low cost, low danger, and few side effects, medicinal herbs are vital in traditional medication all over the world. Traditional therapeutic herb Phyllanthus acidus L. is well-known for its calming possessions on the nervous system. Its roots are known for their activity against bacteria, inflammation, immunity stimulation, and erogenous properties, which contribute to potential health benefits. This study investigates the neuropharmacological effects of P. acidus L. in enhancing memory, targeting cognitive disorders and learning deficits. Analysis of the ethanolic extract’s phytochemistry of P. acidus L. revealed a rich composition of phytoconstituents. UV analysis and thin layer chromatography (TLC) bioassay demonstrated significant acetylcholinesterase (AChE) inhibition by the extract. TLC and high-performance thin-layer chromatography (HPTLC) analyses of the extract indicated distinct and well-separated spots, suggesting the presence of active compounds. Additionally, the extract was assessed for its total phenolic, flavonoid, and ascorbic acid content, revealing antioxidant properties through ex-vivo lipid peroxidation assays. The study concludes that the ethanolic extract of P. acidus L. exhibits memory- enhancing, antidepressant, and neuroprotective effects attributed to its AChE inhibition, modulation of neurotransmitters like dopamine and serotonin, and antioxidant activity. This suggests its potential utility in Alzheimer’s dementia treatment, though further research is necessary to elucidate pharmacology with mechanisms of action and applications.

Keywords: Extraction, Isolation, Neuropharmacological potential, Medicinal plants, Phyllanthus acidus L.

International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.1.43

How to cite this article: Khare N, Tare H, Jain SK. Extraction, Isolation and Neuropharmacological Potential of Phyllanthus acidus L. International Journal of Drug Delivery Technology. 2024;14(1):299-305.

REFERENCES

  1. Ofokansi MN, Ugwah-Oguejiofor CJ, Ihim S, Okoli CO, Akah PA. Neuropharmacological evaluation of the methanol leaf extract of Phyllanthus muellerianus (Kuntze) Exell and its ethyl acetate fraction in mice. Tropical Journal of Pharmaceutical Research. 2021;20(7):1463-72.
  2. Calixto JB, Santos AR, Filho VC, Yunes RA. A review of the plants of the genus Phyllanthus: their chemistry, pharmacology, and therapeutic potential. Medicinal research reviews. 1998 Jul;18(4):225-58.
  3. Hossain M, Akter S, Begum Y, Bulbul I. Analgesic and anti- inflammatory activities of ethanolic leaf extract of Phyllanthus acidus on swiss Albino mice. European Journal of Medicinal Plants. 2016 Jan 10;13(1):1-0.
  4. Santos AR, Valdir Filho C, Yunes RA, Calixto Analysis of the mechanisms underlying the antinociceptive effect of the extracts of plants from the genus Phyllanthus. General Pharmacology: The Vascular System. 1995 Nov 1;26(7):1499-506.
  5. Kassuya CA, Silvestre AA, Rehder VL, Calixto JB. Anti- allodynic and anti-oedematogenic properties of the extract and lignans from Phyllanthus amarus in models of persistent inflammatory and neuropathic pain. European Journal of Pharmacology. 2003 Oct 8;478(2-3):145-53.
  6. Jantan I, Haque MA, Ilangkovan M, Arshad L. An insight into the modulatory effects and mechanisms of action of Phyllanthus species and their bioactive metabolites on the immune system. Frontiers in pharmacology. 2019 Aug 7;10:425684.
  7. Geethangili M, Ding A Review of the Phytochemistry and Pharmacology of Phyllanthus urinaria L. Frontiers in pharmacology. 2018 Oct 1;9:379171.
  8. Rawa MS, Hassan Z, Murugaiyah V, Nogawa T, Wahab HA. Anti-cholinesterase potential of diverse botanical families from Malaysia: Evaluation of crude extracts and fractions from liquid-liquid extraction and acid-base fractionation. Journal of ethnopharmacology. 2019 Dec 5;245:112160.
  9. Kaur N, Kaur B, Sirhindi Phytochemistry and pharmacology of Phyllanthus niruri L.: a review. Phytotherapy research. 2017 Jul;31(7):980-1004.
  10. Uddin MS, Al Mamun A, Iqbal MA, Islam A, Hossain MF, Khanum S, Rashid Analyzing nootropic effect of Phyllanthus reticulatus Poir. on cognitive functions, brain antioxidant enzymes and acetylcholinesterase activity against aluminium- induced Alzheimer’s model in rats: applicable for controlling the risk factors of Alzheimer’s disease. Advances in Alzheimer’s Disease. 2016;5(03):87.
  11. Martini LH, Souza CR, Marques PB, Calixto JB, Yunes RA, Souza DO. Compounds extracted from Phyllantus and Jatropha elliptica inhibit the binding of [3H] glutamate and [3H] GMP-PNP in rat cerebral cortex membrane. Neurochemical 2000 Feb;25:211-5.
  12. Tsakem B, Eckhardt P, Tchuenguem RT, Ponou BK, Dzoyem JP, Teponno RB, Opatz T, Barboni L, Tapondjou LA. Muellerilactone and other bioactive constituents of Phyllanthus muellerianus (kuntze) Biochemical Systematics and Ecology. 2022 Apr 1;101:104397.
  13. Yan X, Li Q, Chen Y, Pan X. Current advances on the phytochemical composition, pharmacologic effects, toxicology, and product development of Phyllanthi fructus. Frontiers in Pharmacology. 2022 Oct 19;13:1017268.
  14. Al Zarzour RH, Ahmad M, Asmawi MZ, Kaur G, Saeed MA, Al-Mansoub MA, Saghir SA, Usman NS, Al-Dulaimi DW, Yam MF. Phyllanthus niruri standardized extract alleviates the progression of non-alcoholic fatty liver disease and decreases atherosclerotic risk in Sprague–Dawley rats. Nutrients. 2017 Jul 18;9(7):766.
  15. Sharif MA, Khan AM, Salekeen R, Rahman MH, Mahmud S, Bibi S, Biswas P, Hasan MN, Islam KM, Rahman SM, Islam Phyllanthus emblica (Amla) methanolic extract regulates multiple checkpoints in 15-lipoxygenase mediated inflammopathies: Computational simulation and in vitro evidence. Saudi Pharmaceutical Journal. 2023 Aug 1;31(8):101681.
  16. Acharya CK, Khan NS, Madhu NR. Medicinal uses of amla, Phyllanthus emblica (Gaertn.): a prospective review. Mukt Shabd Journal. 2021;10(10):296-310.
  17. Prananda AT, Syahputra RA, Nurkolis F. Phyllanthus emblica: a comprehensive review of its phytochemical composition and pharmacological properties. Frontiers in Pharmacology. 2023 Oct 26;14:1288618.
  18. Hasan MR, Islam M N, Islam Phytochemistr y, pharmacological activities and traditional uses of Emblica officinalis: A review. International Current Pharmaceutical Journal. 2016 Jan 18;5(2):14-21.
  19. Sonawane A, Jawale G, Devhadrao N, Bansode A, Lokhande J, Kherade D, Sathe P, Deshmukh N, Dama G, Tare H. Formulation And Development Of Mucoadhesive Nasal Drug Delivery Of Ropinirol Hcl For Brain Int J App Pharm. 2023;15(5):325-32.
  20. Mane VB, Killedar SG, More HN, Tare HL, Evaluation of acute oral toxicity of the Emblica officinalis Phytosome Formulation in Wistar International Journal of Drug Delivery Technology. 2022;12(4):1566-1570.
  21. Singh A, Singh L, Pandey R, Singh P, Ali M, Kaushik R, Soni P. Neuroprotective Effect of Chlorogenic Acid against Pentylenetetrazol Induced Kindled Epilepsy in Mice. International Journal of Drug Delivery Technology. 2023;13(3):1030-1036.
  22. Baqir KA, Selman SM,Mohammed SB. Neuroprotective Effects of Taraxicum Officinale as an Antioxidant and Anti- neuroinflammatory Agent in Rotenone Induced Rat Model of Parkinson’s Disease. International Journal of Drug Delivery Technology. 2021;11(3):649-655.