Formulation Optimization and Preparation of Amlodipine and Telmisartan Double-layer Tablets (5/40 Mg) using Wet Granulation Method
Duyen Thi My Huynh1, Huynh Thien Hai1, Tran Huu Loc1, Minh-Ngoc T Le1, Phuoc-Vinh Nguyen2*
1Can Tho University of Medicine and Pharmacy
2School of Medicine, Vietnam National University Ho Chi Minh City
Received: 09th May, 2024; Revised: 25th May, 2024; Accepted: 05th June, 2024; Available Online: 25th June, 2024
ABSTRACT
The two-layer tablet of amlodipine and telmisartan is an effective strategy to manage hypertension disease through a combination of various treatment mechanisms. However, the spray-drying method is currently used to prepare telmisartan tablets due to its extremely low solubility, which requires sophisticated equipment. In this study, wet granulation was applied to fabricate this two-layer tablet. Furthermore, design of experiments were deployed to design and optimize the formulation in order to obtain an in-vitro equivalence with the reference drug. As a result, a formulation of the two-layer table using the wet granulation method was successfully developed and optimized. The obtained tablet showed an in-vitro equivalence with the reference drug (with f2 superior to 50 for both amlodipine and telmisartan). Furthermore, the obtained tablet also met a set of in-house standards, including appearance, identification, assay, and dissolution rate. This study provided not a novel but effective and simple method of fabrication for amlodipine-telmisartan tablets using wet granulation. Furthermore, the obtained results also highlighted the significant contribution of a well-established design of experiments in the development of a complex tablet formulation.
Keywords: Amlodipine, Telmisartan, Two-layer tablets, In-vitro equivalence, Wet granulation, Design experiment. International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.2.32
How to cite this article: Huynh DTM, Hai HT, Loc TH, Le MT, Nguyen P. Formulation Optimization and Preparation of Amlodipine and Telmisartan Double-layer Tablets (5/40 Mg) using Wet Granulation Method. International Journal of Drug Delivery Technology. 2024;14(2):810-826.
REFERENCES
- Kasture A, Ramteke M. Simultaneous UV-spectrophotometric method for the estimation of atenolol and amlodipine besylate in combined dosage Indian J Pharm Sci. 2006;68(3). Available from: doi: 10.4103/0250-474X.26665
- Organization WH. Guideline for the pharmacological treatment of hypertension in adults. World Health Organization; 2021.
- Elliott WJ, Ram CVS. Calcium channel blockers. J Clin 2011;13(9):687. Available from: doi: 10.1111/j.1751- 7176.2011.00513.x. Epub 2011 Jul 27.
- Taylor AA, Siragy H, Nesbitt Angiotensin Receptor Blockers: Pharmacology, Efficacy, and Safety: Angiotensin Receptor Blockers. J Clin Hypertens. 2011;13(9):677–86. Available from: doi: 10.1111/j.1751-7176.2011.00518.x. Epub 2011 Jul 27.
- Ministry of Health of Vietnam. Vietnamese National Drug Formulary. 2018.
- Xie M, Tang T, Liang Efficacy of single‐pill combination in uncontrolled essential hypertension: A systematic review and network meta‐analysis. Clin Cardiol. 2023;46(8):886–98. Available from: doi: 10.1002/clc.24082.
- The Patent Cooperation Treaty. Bilayer tablet comprising Telmisartan and Amlodipine. 2006.
- ICH Harmonised Guideline. Q2(R1). Validation of Analytical procedures text and methodology. 2022.
- Kottai MA, Sankhla R, Gupta S, Smith AA, Manavalan R. Development and validation of a reversed phase HPLC method for simultaneous determination of amlodipine and telmisartan in pharmaceutical dosage J. Appl. Chem. Res. 2010;12:43-
- Maimoon S, Reddy KNK, Swetha P. RP-HPLC method development and validation for simultaneous estimation of Amlodipine besylate and Telmisartan in tablet dosage form. Indian J Res Pharm Biotechnol. 2017;5(1):74-76.
- Patra BR, Mohan S, Gowda N. International conference on harmonization recommended forced degradation studies and development of a new validated isocratic reverse-phase ultra high-performance liquid chromatography method for the simultaneous estimation of telmisartan and amlodipine in bulk drug and marketed formulation. Asian J Pharm Clin Res. 2019;12(6):250–258. Available from: doi: 22159/ajpcr.2019. v12i6.32629.
- Kayillo S, Dennis GR, Shalliker RA. Retention of polycyclic aromatic hydrocarbons on propyl-phenyl stationary phases in reversed-phase high performance liquid chromatography. J Chromatogr 2007;1148(2):168–76. Available from: https://doi. org/10.1016/j.chroma.2007.03.004.
- Kotamarthy L, Dan A, Karkala S, Parvani S, Román-Ospino AD, Ramachandran R. Twin-screw granulation: Mechanistic understanding of the effect of material properties on key granule quality attributes through the analysis of mixing dynamics and granulation rate mechanisms. Adv Powder Technol. 2023;34(9):104137. Available from: https://doi.org/10.1016/j. 2023.104137.
- Van der Merwe J, Steenekamp J, Steyn D, Hamman The role of functional excipients in solid oral dosage forms to overcome poor drug dissolution and bioavailability. Pharmaceutics. 2020;12(5):393. Available from: doi: 10.3390/ pharmaceutics12050393.
- Huynh DTM, Tran VH, Le MNT, Huynh VH, Pham Floating tablets incorporating curcumin solid dispersion as a potential pharmaceutical dosage form for stomach cancer treatment. J Appl Pharm Sci. 2023;13(4):240–50. Available from: doi: 10.7324/JAPS.2023.114417.
- Huynh DTM, Hai HT, Hau NM, Lan HK, Vinh TP, De Tran V, et Preparations and characterizations of effervescent granules containing azithromycin solid dispersion for children and elder: Solubility enhancement, taste-masking, and digestive acidic protection. Heliyon. 2023;9(6): e16592. Available from: doi: 10.1016/j.heliyon.2023.e16592.