International Journal of Drug Delivery Technology
Volume 14, Issue 2

Application of PCR and ELISA for Investigation the Helicobacter pylori in Stool Specimens of Infected Patients

Saja A Al-Jumaili1*, Amina N Althwani1, Ahmed Dawood Ahmed2, Ahmad Abd alraheem Ibrahem Dahy3, Zina Najim1, Nawal M Al-Khalidi4

1Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, University of Baghdad, Baghdad, Iraq.

2Bilad Alrafidain University Collage, Iraq.

3Mustansiriyah University, Iraq.

4Gastroenterology and Hepatology Teaching Centre, Ministry of Health, Iraq. 

Received: 12th October, 2023; Revised: 28th November, 2023; Accepted: 05th December, 2023; Available Online: 25th June, 2024 

ABSTRACT

Background: Infection with Helicobacter pylori is a global health issue, and non-invasive and accurate testing is a promising diagnostic method. Because H. pylori antibodies collapse quite gradually even after effective treatment. So, the tests for identifying the antibody of H. pylori are missing specificity and sensitivity. Instead, the tests of stool antigen for H. pylori are described as an alternative method because they have more reliability and simplicity.

Aim: The study was aimed to assess the performance of enzyme-linked immunosorbent assays (ELISA), for the detection of E. pylori antigen in stool and compare it to molecular tests by using two housekeeping genes.

Methods: Stool samples were collected from patients (85) who attended the Gastroenterology and Hepatology Teaching Center in Baghdad, Iraq, and underwent esophagogastroduodenoscopy (EGD) for biopsy in a period extended from November 2020 to July 2021. PCR and ELISA were used to Investigate H. pylori in stool specimens of infected patients.

Results: The result revealed that 74/85 (87.88%) of specimens were positive by ELISA, whereas 49/85 (57.6%) were positive for the 16 SrRNA gene related to Helicobacter genus and 35/85 (41.1%) were positive for amiA gene related to H. pylori species, and that means the ELISA test is more accurate than PCR analysis for detection of H. pylori bacteria in stool specimen.

Conclusion: Detection H. pylori Ag using the ELISA test was superior to the molecular test. Therefore, the ELISA test might replace other methods and could be used for the detection of active H. pylori infection before the inauguration of treatment between dyspeptic patients.

Keywords: Helicobacter pylori, 16S rRNA gene , AmiA gene, ELISA , Stool antigen. International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.2.68

How to cite this article: Al-Jumaili SA, Althwani AN, Ahmed AD, Dahy AAAI, Najim Z, Al-Khalidi NM. Application of PCR and ELISA for Investigation the Helicobacter pylori in Stool Specimens of Infected Patients. International Journal of Drug Delivery Technology. 2024;14(2):1055-1058.

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