International Journal of Drug Delivery Technology
Volume 14, Issue 3

Impact of Nanoethosomes Loaded with Polyherbal Extracts on the Mechanisms Involved in Wound Healing In-vitro Research

Mekala K, Shaheedha SM*, Prakash R

Crescent School of Pharmacy, B. S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, Tamilnadu, India

Received: 24th January, 2024; Revised: 24th April, 2024; Accepted: 16th May, 2024; Available Online: 25th September, 2024

ABSTRACT

This study focuses on formulating and optimizing Polyherbal Nanoethosomes for controlled drug delivery, utilizing a polyherbal extract comprising Basella alba L., Portulaca oleracea L., Lawsonia inermis L., Trigonella foenum L., and Peristrophe paniculata L. Comprehensive characterization revealed a variety of secondary metabolites within the extract. The primary goal was to create poly herbal extract-entrapped ethosomes that could be integrated into a sustained-release transdermal gel dosage. We prepared nanoethosomes using phosphatidylcholine, ethanol, Tween 80, and SLS, and optimized them using Design Expert software. The characteristics included size (particle size: 121 ± 2.60 nm) and entrapment efficiency (81.91 ± 1.92%), followed by incorporation into a carbopol gel for skin application. We performed in-vitro permeation studies and cytotoxic assays on L-929 cells, which revealed favorable outcomes. Optimized nanoethosomes displayed desirable particle characteristics, highlighting their potential for controlled drug delivery. The nano vesicular gels had good rheological and physical properties (polydispersity index: 0.41 ± 0.04, zeta potential: -8.12 ± 1.25 mV), which meant they could be used topically. This research underscores the compatibility of plant extracts with formulation components, offering a foundation for Polyherbal Nanoethosomes in pharmaceutical formulations. The study emphasizes precise control over particle attributes and suggests potential applications in enhancing drug release and bioavailability.

Keywords: Polyherbal nanoethosomes, Controlled drug delivery, Transdermal gel, optimization. International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.12

How to cite this article: Mekala K, Shaheedha SM, Prakash R. Impact of Nanoethosomes Loaded with Polyherbal Extracts on the Mechanisms Involved in Wound Healing In-vitro Research. International Journal of Drug Delivery Technology. 2024;14(3):1334-1343.

REFERENCES

  1. Soni H, Mishra K, Sharma S, Singhai AK. Characterization of Azadirachtin from ethanolic extract of leaves of Azadirachta Journal of Pharmacy Research. 2012 Jan;5(1):199-201.
  2. Menna C, Calista N, Aurino L, Dwijayanti AD. Aloe vera vs. Silver sulfadiazine for treating second–degree burn wounds: Evidence-based case report. International Journal of Applied 2019;11:146-148.
  3. Gayathiri K, Chitra In-vitro, In-vivo Study of Jatropha curcas Leaves Extract, and Preparation of a Nanoemulsion by a Low- energy/Solvent-free Method, In-silico Study of β-Sitosterol in Ulcerative Colitis. International Journal of Drug Delivery Technology, 2024;14(1):265-273. DOI: 10.25258/ijddt.14.1.38
  4. Martin P, Leibovich SJ. Inflammatory cells during wound repair: the good, the bad and the Trends in cell biology. 2005;15(11):599-607. DOI: https://doi.org/10.1016/j. tcb.2005.09.002
  5. Pierce GF, Yanagihara D, Klopchin K, Danilenko DM, Hsu E, Kenney WC, Morris CF. Stimulation of all epithelial elements during skin regeneration by keratinocyte growth factor. The Journal of experimental medicine. 1994;179(3):831-840. DOI: https://doi.org/10.1084/jem.179.3.831
  6. Gillis P, Savla U, Volpert OV, Jimenez B, Waters CM, Panos RJ, Bouck Keratinocyte growth factor induces angiogenesis and protects endothelial barrier function. Journal of cell science. 1999;112(12):2049-2057. DOI: https://doi.org/10.1242/ jcs.112.12.2049
  7. Sun T, McMinn P, Coakley S, Holcombe M, Smallwood R, MacNeil S. An integrated systems biology approach to understanding the rules of keratinocyte colony formation. Journal of the Royal Society Interface. 2007;4(17):1077-1092. DOI: https://doi.org/10.1098/rsif.2007.0227
  8. Obara K, Sumi K, Fukuda H. The use of multiple transcription starts causes the dual targeting of Arabidopsis putative monodehydroascorbate reductase to both mitochondria and Plant and cell physiology. 2002;43(7):697-705. DOI: https://doi.org/10.1093/pcp/pcf103
  9. Efferth T, Kahl S, Paulus K, Adams M, Rauh R, Boechzelt H, Hao X, Kaina B, Bauer R. Phytochemistry and pharmacogenomics of natural products derived from traditional Chinese medicine and Chinese materia medica with activity against tumor cells. Molecular Cancer 2008;7(1):152-161. DOI: https:// doi.org/10.1158/1535-7163.MCT-07-0073
  10. Goswami M, Kulshreshtha M, Rao CV, Yadav S, Yadav Anti- ulcer potential of Lawsonia inermis L. leaves against gastric ulcers in rats. Journal of Applied Pharmaceutical Science. 2011:69-72. DOI: https://japsonline.com/abstract.php?article_ id=23&sts=2
  11. Raghav S Shraddha, Bhavna Kumar, Sethiya K Neeraj, Singhal Manmohan, Arya Swati. In-vitro Analysis of Antioxidant, Anti-inflammatory, Antidiabetic and Antimicrobial Studies of Kaempferol Ethosomes, International Journal of Drug Delivery Technology. 2024;14(1):118-125. DOI: 25258/ijddt.14.1.17
  12. Hardy A, Poulos A, Emanuel N, Reed An investigationof advanced practice carried out by radiographers in New South Wales. Radiographer. 2010;57(3):29-33. DOI: https://doi. org/10.1002/j.2051-3909.2010.tb00134.x
  13. Keenan LY, Muir C, Cuthbertson Maximizing the benefit– minimizing the risk: the developing role of radiographers in performing intravenous injections. The British Journal of Radiology. 2001;74(884):684-689. DOI: https://doi.org/10.1259/ bjr.74.884.740684
  14. Azad AK, Wan Azizi WS, Babar ZM, Labu ZK, Zabin S. An overview of phytochemical, Anti-inflammatory and Antibacterial activity of Basella alba leaves extract. Middle-East Journal of Scientific Research. 2013;14(5):650-655. DOI: 10.5829/idosi. 2013.14.5.71225
  15. Selvakumar S, Vimalanban S, Balakrishnan G. Quantitative determination of phytochemical constituents from Anisomeles MOJ Bioequivalence Bioavailability. 2019;6(2):19-21. DOI: https://medcraveonline.com/MOJBB/MOJBB-06-00130.pdf
  16. Pathan IB, Jaware BP, Shelke S, Ambekar W. Curcumin loaded ethosomes for transdermal application: Formulation, optimization, in-vitro and in-vivo study. Journal of Drug Delivery Science and 2018;44:49-57. DOI: https:// doi.org/10.1016/j.jddst.2017.11.005
  17. Panda S, Suryawanshi M. Fabrication, Characterization and Toxicity Evaluation Chemically Cross Linked Polymeric Material: A Proof of Concept. International Journal of Pharmaceutical Sciences and Nanotechnology (IJPSN). 2023;16(3):6522-6532. DOI: https://doi.org/10.37285/ijpsn.2023.16.3.6
  18. Nayak D, Tawale RM, Aranjani JM, Tippavajhala VK. Formulation, Optimization and Evaluation of Novel Ultra- deforwsxmable Vesicular Drug Delivery System for an Antifungal Drug. AAPS PharmSciTech. 2020;21(5):1-10. DOI: https://doi.org/10.1208/s12249-020-01681-5
  19. Shobana G, John NA, Rameshkannan Quantitative Analysis of Physicochemical and Phytochemical Constituents Medicinal Herbs. Journal of Stress Physiology & Biochemistry. 2023;19(3):79-86. DOI: https://cyberleninka.ru/article/n/ quantitative-analysis-of-physicochemical-and-phytochemical- constituents-medicinal-herbs
  20. Ibrahim TA, Ajongbolo KF, Aladekoyi G. Phytochemical Screening and Antimicrobial Activity of Crude Extracts of Basella alba and Helianthus annuus on Selected Food Research & Reviews: Journal of Microbiology and Biotechnology. 2014;3(2):27-31. DOI: https://www.semanticscholar.org/paper/ Phytochemical-Screening-and-Antimicrobial-Activity-Ta-Kf/1 f4d500488126b24d94cf9f410e11ee140492412
  21. Adedeji GA, Ogunsanwo OY, Elufioye Quantifications of phytochemicals and biocide actions of Lawsonia inermis linn. Extracts against wood termites and fungi. International Biodeterioration & Biodegradation. 2017;116:155-162. DOI: https://doi.org/10.1016/j.ibiod.2016.10.026
  22. Mahmood NM, Yahya KI. Nutrient and phytochemical of fenugreek (Trigonella foenum graecum) seeds. International Journal of Sciences: Basic and Applied 2017;36(3):203-DOI: http://gssrr.org/index.php?journal=JournalOfBasicA ndApplied
  23. Keser S, Celik S, Turkoglu S, Yilmaz O, Ismail T. Antioxidant activity, total phenolic and f lavonoid content of water and ethanol extracts from Achillea millefolium Turkish Journal of Pharmaceutical Sciences. 2013;10(3):385–392. DOI: https://cms. galenos.com.tr/Uploads/Article_12377/385-392.pdf
  24. Eghdami A, Sadeghi Determination of total phenolic and f lavonoids contents in methanolic and aqueous extract of Achillea millefolium. Organic Chemistry Journal. 2010;2:81-84. DOI:https://api.semanticscholar.org/CorpusID:96668196
  25. Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic Journal of agricultural and food chemistry. 1999;47(10):3954-3962. DOI: https://pubs.acs.org/doi/ abs/10.1021/JF990146L
  26. Poojary MM, Passamonti Optimization of extraction of high purity all-trans-lycopene from tomato pulp waste. Food Chemistry. 2015;188:84-91. DOI: https://doi.org/10.1016/j. foodchem.2015.04.133
  27. Liu F, Ng Antioxidative and free radical scavenging activities of selected medicinal herbs. Life sciences. 2000;66(8):725-735. DOI: https://doi.org/10.1016/S0024-3205(99)00643-8
  28. Muruganantham S, Anbalagan G, Ramamurthy N. FT-IR and SEM-EDS comparative analysis of medicinal plants, Eclipta alba Hassk and Eclipta prostrata Romanian Journal of Biophysics. 2009;19(4):285-294. DOI: https://www.rjb.ro/articles/259/smur.pdf
  29. Sahu AR, Bothara Formulation and evaluation of phytosome drug delivery system of boswellia serrata extract. International Journal of Research in Medical Sciences. 2015;4(2):94–99. DOI: 143746657719_Asit_20Sahu_20.pdf-libre.pdf
  30. Ascenso A, Raposo S, Batista C, Cardoso P, Mendes T, Praça FG, Bentley MV, Simões Development, characterization,and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes. International journal of nanomedicine. 2015:5837-5851. DOI: https://www. tandfonline.com/doi/full/10.2147/IJN.S86186
  31. Danaei MR, Dehghankhold M, Ataei S, Hasanzadeh Davarani F, Javanmard R, Dokhani A, Khorasani S, Mozafari Impact of particle size and polydispersity index on the clinical applications of lipidic nanocarrier systems. Pharmaceutics. 2018;10(2):57. DOI: https://doi.org/10.3390/pharmaceutics10020057
  32. Maya S, Sabitha M, Nair SV, Jayakumar R. Phytomedicine- loaded polymeric nanomedicines: potential cancer therapeutics. Multifaceted Development and Application of Biopolymers for Biology, Biomedicine and Nanotechnology. 2013:203-239. DOI: https://doi.org/10.1007/12_2012_195
  33. Gnana RPM, Devhare LD, Dharmamoorthy G, Khairnar MV, Prasidha R. Synthesis, Characterisation, Molecular Docking Studies and Biological Evaluation of Novel Benzothiazole Derivatives as EGFR Inhibitors for Anti-breast Cancer Agents. International Journal of Pharmaceutical Quality Assurance. 2023;14(3):475-480. DOI: 10.25258/ijpqa.14.3.03
  34. Priya MGR, Prasanth LML, Devhare LD, Yazdan SK, Gunjal S. Synthesis, DNA Binding, Molecular Docking and Anticancer Studies of Copper (II), Nickel (II), and Zinc (II) Complexes of Primaquine-based Ligand. International Journal of Pharmaceutical Quality Assurance. 2024;15(1):69-75. DOI: 25258/ijpqa.15.1.10