International Journal of Drug Delivery Technology
Volume 14, Issue 3

In-vitro antidiabetic activity of ethanolic extract from Khla (Schumannianthus dichotomus Roxb.) rhizome

Yuttana Sudjaroen*, Kanittada Thongkao, Pimporn Thongmuang

Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok, Thailand. 

Received: 24th January, 2024; Revised: 20th April, 2024; Accepted: 16th June, 2024; Available Online: 25th September, 2024 

ABSTRACT

Schumannianthus dichotomus Roxb. in Thai, namely “Khla,” is notably distributed in South and South-East Asia regions. In Thai traditional medicine, rhizome has a bland taste, and after decoction, it is used to reduce body heat and fever and treatment of skin diseases. Recently, the methanolic extract of rhizome had reported the hepatoprotective and hypoglycemic activities in mice. Aims of this study were conducted to determine the nutritional value of rhizome and to evaluate α-glucosidase, and α-amylase inhibitory activities of ethanol extract from the rhizome (KE).

The rhizome had a high content of dietary fiber and iron, while it was low in calories, fat, and carbohydrates. Thus, it can become an ingredient of a healthy diet. The total phenolic content of KE contained 229.8 ± 6.23 mg of gallic acid equivalent (GAE)/g. There was strongly inhibited α-glucosidase (IC50 = 0.11 ± 0.03 mg/mL) and comparable with the antidiabetic drug acarbose (IC50 = 0.10 ± 0.03 mg/mL). Therefore, KE was slightly inhibited by α-amylase (IC50 = 0.42 ± 0.05 mg/mL), when compared with acarbose (IC50 = 0.016 ± 0.001 mg/mL). Thus, the phytochemicals of different extracts are necessary to be identified. Therefore, the advance techniques were the limitation of our study.

Keywords: Schumannianthus dichotomus Roxb., Antidiabetes, α-Glucosidase inhibition, α-Amylase inhibition. International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.02

How to cite this article: Sudjaroen Y, Thongkao K, Thongmuang P. In-vitro antidiabetic activity of ethanolic extract from Khla (Schumannianthus dichotomus Roxb.) rhizome. International Journal of Drug Delivery Technology. 2024;14(3):1257-1260.

REFERENCES

  1. Kumar S, Mittal A, Babu D, Mittal A. Herbal Medicines for Diabetes Management and its Secondary Complications. Curr Diabetes Rev 2021;17(4):437–56. doi.org/10.2174/157339981666 6201103143225
  2. Maneenoon K, Khuniad C, Teanuan Y, Saedan N, Prom-in S, Rukleng N, et al. Ethnomedicinal plants used by traditional healers in Phatthalung Province, Peninsular J Ethnobiol Ethnomed 2015; 11(43): 1-20.doi.org/10.1186/s13002-015-0031-5
  3. Ahmed R, Islam ANMF, Rahman M, Halim Management and economic value of Schumannianthus dichotoma in rural homesteads in the Sylhet region of Bangladesh. Int J Biodivers Sci Ecosyst Serv 2007; 3(4): 252–8. doi. org/10.1080/17451590709618178
  4. Veldkamp JF, Turner The correct name for Schumannianthus dichotomus (Marantaceae). Kew Bull 2016; 71: 1-4. doi. org/10.1007/S12225-016-9660-7
  5. Sajib NH, Uddin SB. Ethnomedicinal Study of Plants in Hathazari, Chittagong, Pertanika journal of tropical agricultural science Pertanika J Trop Agric Sci 2015; 38(2): 197-210.
  6. Basher MA, Hoque MdR, Roy R, Hosen S, Karim I, Bhowmik T, et Sex-dependent variations in antinociceptive and antipyretic effects of rhizome and stem extract of Schumannianthus dichotomus Roxb. in male and female mice. Indonesian J Pharm 2020; 31(4): 323-34. doi.org/10.22146/ijp.602
  7. Akter A, Roy R, Basher MA. In-vivo hepatoprotective and hypoglycemic effects of methanolic extract of Schumannianthus dichotomus Phytomedicine Plus 2023; 3(3): 100459.https://doi.org/10.1016/j.phyplu.2023.100459
  8. Department of Fine Textbook of Medicine, Royal Issue of Rama V, Vol. 2. 1999, Bangkok: Amarin Printing and Publishing Public Company. (in Thai).
  9. Official Methods of Analysis of the Association of Official Analytical Chemists: Official Methods of Analysis of AOAC International (21st ed.) 2019, Washington DC: AOAC.
  10. Bureau of Nutrition, Department of Health, Ministry of Public Health. Dietary reference intake for Thais 2020, Bangkok: Ministry of Public Health.
  11. Hussein EA, Taj-Eldeen AM, Al-Zubairi AS, Elhakimi AS, Al-Dubaie AR. Phytochemical Screening, Total Phenolics and Antioxidant and Antibacterial Activities of Callus from Brassica nigra Hypocotyl Explants. Int J Pharmacol 2010; 6(4): 464–71. doi.org/10.3923/ijp.2010.464.471
  12. Alam MdA, Zaidul ISM, Ghafoor K, Sahena F, Hakim MdA, Rafii MY, et In vitro antioxidant and, α-glucosidase inhibitory activities and comprehensive metabolite profiling of methanol extract and its fractions from Clinacanthus nutans. BMC Complement Altern Med 2017; 17(1): 181. doi.org/10.1186/ s12906-017-1684-5
  13. Keerthana G, Kalaivani MK, Sumathy In vitro alpha-amylase inhibitory and antioxidant activities of ethanolic leaf extract of Croton bonplandianum. Asian J Pharm Clin Res 2013; 6(8): 32-6.
  14. Jin DX, He JF, Luo XG, Zhang TC. Hypoglycemic effect of Hypericum attenuatum Choisy extracts on type 2 diabetes by regulating glucolipid metabolism and modulating gut microbiota. J Funct Foods 2019; 52: 479–91. doi.org/10.1016/j.jff.2018.11.031
  15. Reza MS, Shuvo MSR, Hassan MM, Basher MA, Islam MA, Naznin NE, et al, 2020. Antidiabetic and hepatoprotective potential of whole plant extract and isolated compounds of Aeginetia indica. Biomed Pharmacother 2020; 132: doi. org/10.1016/j.biopha.2020.110942
  16. Marchyshyn S, Slobodianiuk L, Budniak L, Ivasiuk Hypoglycemic effect of Cyperus esculentus L. tubers extract. Pharmacologyonline 2021; 2: 1383–92. https://doi. org/10.1155/2020/8856129
  17. Piragine E, Flori L, Di Cesare Mannelli L, Ghelardini C, Pagnotta E, Matteo R, et Eruca sativa Mill. seed extract promotes anti- obesity and hypoglycemic effects in mice fed with a high-fat diet. Phytother Res 2021; 35: 1983–90. https://doi.org/10.1002/ptr.6941