International Journal of Drug Delivery Technology
Volume 14, Issue 3

Development and Characterization of Gingerol-Assisted Silver Nanoparticle and Chitosan Alginate Membrane for Antibacterial and Wound Healing Activity

Manisha Khaire, Papiya Bigoniya*

DSKM College of Pharmacy, Faculty of Pharmacy, RKDF University, Bhopal, Madhya Pradesh, India. 

Received: 08th March, 2024; Revised: 26th May, 2024; Accepted: 02nd August, 2024; Available Online: 25th September, 2024 

ABSTRACT

This study synthesized and characterized gingerol-loaded silver nanoparticles (Ag-NPs), which were incorporated into chitosan-alginate (Cs/Alg) films for advanced wound care applications. Gingerol was isolated with a 9.81% yield, forming pale-yellow needle-shaped crystals with a melting point of 32ºC. The purity and structure of gingerol were confirmed through thin-layer chromatography (TLC), UV-visible, fourier-transform infrared spectroscopy (FTIR), and high-performance liquid chromatography (HPLC) analyses. The synthesized Ag-NPs, particularly from the GN3 batch, exhibited a small particle size (36.52 ± 3.52 nm), high stability (zeta potential of -23.20 mV), and notable encapsulation efficiency (76.35%) and yield (74.11%). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses revealed their spherical and smooth morphology. Stability tests over three months showed no substantial changes in particle size, ZP, or %EE. The Cs/Alg films demonstrated appropriate thickness, weight, water uptake, and pH changes, with GN-CM4 showing the best performance. In-vitro drug release studies indicated increased release rates, especially for GN-CM4, which also exhibited the highest antibacterial activity. In-vivo studies on excision and burn wound models showed that GN-CM4 promoted complete wound closure by day 21, enhanced hydroxyproline levels, reduced myeloperoxidase content, and facilitated superior tissue regeneration. These findings underscore the potential of gingerol-loaded Ag-NPs in enhancing wound healing through their anti-inflammatory and antibacterial properties.

Keywords: Gingerol, Silver nanoparticles, Chitosan alginate (Cs/Alg) film, Infected incision wound, Burn wound, wound

healing

International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.29

How to cite this article: Khaire M, Bigoniya P. Development and Characterization of Gingerol-Assisted Silver Nanoparticle and Chitosan Alginate Membrane for Antibacterial and Wound Healing Activity. International Journal of Drug Delivery Technology. 2024;14(3):1456-1468.

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