International Journal of Drug Delivery Technology
Volume 14, Issue 3

Bioguided Fractionation and Isolation of Natural Antidiabetic Agents from Polyherbal Tablet

Sunayana R Vikhe1*, Sharvari V Vikhe1, Rahul L Vikhe2, Vilas B Ghawate3

1Department of Pharmacognosy, Pravara Rural College of Pharmacy Loni, Maharashtra India.

2Department of Financial management, Sir Vishveshwaraiya Institute of Technology, Sinnar, Maharashtra India.

3Department of Pharmacognosy, Mula Education society’s college of Pharmacy, Sonai, Maharashtra, India.

 Received: 25th March, 2024; Revised: 08th July, 2024; Accepted: 06th August, 2024; Available Online: 25th September, 2024

ABSTRACT

Polyherbal antidiabetic tablets were formulated, evaluated and compressed by combining the powder extract of Nigella sativa, Trigonella foenum, and Glycyrrhiza Glabra. A number of tests were carried out, including a uniformity weight test, an assessment of the tablets’ hardness and friability, an ash value, moisture content, an extractive value that is soluble in water or alcohol, density (bulk and tapped), repose angle, Carr’s index, disintegration time with preliminary phytochemical screening. The tablet also had ex-vivo antidiabetic performance to enzymes alpha-amylase as well as glucosidase. To ensure the presence of active ingredients, thin-layer chromatography was performed. Column chromatography as performed using silica gel G as a stationary phase. The column of material was created using acetic acid, toluene, and ethyl acetate. As follows: (5.0:4.2:0.8) for 10 ml. The mobile phase of the sample, which contains toluene, ethyl acetate, acetic acid (50:42:8) and fractions, was isolated, collected and sent to IIT Bombay for GCHRMS analysis. In GCHRMS, the chemical constituent was found to be 2 H-Pyran-2-one, tetrahydro-4-hydroxy-4-methyl-, Tetrahydro [2 , 2’] bifuranyl-5-one, 1H-Benzimidazole, 2-(1-methylethyl)-, Bifenthrin, Propionamide, 2,2-diphenyl-N-(2-pyridinyl)-, Dihydromyristicin, 2-Dodecanone, di-p-Tolyl sulfone. The tablets showed promising results for alpha-amylase and alpha-glucosidase inhibition activities.

Keywords: Antidiabetic, evaluation, compression, polyherbal tablets, alpha amylase, alpha glucosidase, active constituents, GCHRMS

International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.35

How to cite this article: Vikhe SR, Vikhe SV, Vikhe RL, Ghawate VB. Bioguided Fractionation and Isolation of Natural Antidiabetic Agents from Polyherbal Tablet. International Journal of Drug Delivery Technology. 2024;14(3):1511-1515.

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