International Journal of Drug Delivery Technology
Volume 14, Issue 3

An Approach to Development and In-vivo Evaluation of Beta Sitosterol Phytosomes by Taguchi Orthogonal Array Design

Srikala Kamireddy1,2, S Sangeetha1*, Harekrishna Roy2*

1SRM College of Pharmacy, Chennai, Tamil Nadu, India

2 Nirmala college of pharmacy, Mangalgiri, Andhra Pradesh, India. 

Received: 07th March, 2024; Revised: 04th May, 2024; Accepted: 15thJuly, 2024; Available Online: 25th September, 2024 

ABSTRACT

The research mainly focused on developing and optimizing beta-sitosterol phytosomes through a systematic Taguchi orthogonal array design and assessment of their performance in both beta-sitosterol phytosomes. The fabrication of phytosomes containing β-sitosterol was carried out using the rotary evaporation technique. An orthogonal array design was employed in the study to precisely optimize the phytosomes. In this two responses and three independent variables were analyzed. The data was analyzed by using statistical modeling. Characterization of these phytosomes involves Fourier transform infrared spectroscopy, entrapment efficiency, differential scanning calorimetry compatibility study, zeta potential and particle size. Based on the findings, an optimized phytsome formulation was developed to achieve the best results. The results indicated that beta-sitosterol phytosomes responses are significant. It effectively reduced body weight as well as levels of total cholesterol (126.120 ± 0.98 mg/dl), triglycerides (104.23 ± 2.8 mg/dl), HDL (39.31 ± 0.45 mg/dl), VLDL (21.67 ± 0.34 mg/dl), and LDL (76.43 ± 0.34 mg/dl) in rats compared to the other groups. The orthogonal array design study observed that the anti-hyperlipidemic activity of β-sitosterol was nearly as potent as that achieved with standard drug therapy.

Keywords: Beta-sitosterol, phytosome, Taguchi orthogonal array design, total cholesterol, Triglycerides

International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.58

How to cite this article: Kamireddy S, Sangeetha S, Roy H. An Approach to Development and In-vivo Evaluation of Beta Sitosterol Phytosomes byTaguchi OrthogonalArray Design. International Journalof Drug DeliveryTechnology. 2024;14(3):1661-1667.

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