International Journal of Drug Delivery Technology
Volume 14, Issue 3

Development and Characterization of Ethanolic Extract of Cleome gynandra L. Loaded Solid Lipid Nanoparticles for the Management of Diabetes Mellitus

Abarnadevika A1,2, Ariharasivakumar G2, Anusha D3, Nagalakshmi S1*

1Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India.

2Department of Pharmacology, KMCH College of Pharmacy, Coimbatore, Tamil Nadu, India.

3Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Chennai, Tamil Nadu, India. 

Received: 20th February, 2024; Revised: 27th March, 2024; Accepted: 15th August, 2024; Available Online: 25th September, 2024 

ABSTRACT

The bioactive components of the botanical remedy Cleome gynandra L., which is famous for its antidiabetic effects, were extracted using ethanol. To improve its stability, bioavailability, and therapeutic potential, the extract was subsequently encapsulated within solid lipid nanoparticles (SLNs) using a high-pressure homogenization method with varying concentrations of lipids. Zeta potential, entrapment efficiency, kinetics of drug release, and particle size were among the numerous characteristics of the SLNs that were thoroughly examined. One important measure for determining whether bioactive compounds were successfully encapsulated within the SLNs is the entrapment efficiency. An impressive entrapment efficiency of more than 82.13% was shown by the optimized formulation F4. The optimized formulation F4 exhibited a mean particle size ranging from 243.5 to 261.7 nm, according to particle size analysis. This size range is ideal for improving cellular uptake and bioavailability. A negative charge was observed in the zeta potential measurements, which means that the SLNs are stable and do not aggregate. A PDI of 0.416 provided additional evidence of the nanoparticles’ stability and uniformity. A sustained release of about 62.57% over 7 hours was shown in the in-vitro drug release profile data, indicating a long-lasting therapeutic effect. Improved therapeutic efficacy of antidiabetic treatment and more stable blood glucose levels are both benefits of the sustained release profile. The in-vitro results of formulation F4 suggest that SLNs have the good stability, bioavailability, and therapeutic potential of C. gynandra L.’s ethanolic extract when used as a delivery system.

Keywords: Cleome gynandra L., Solid lipid nanoparticles, Ethanolic extract, Antidiabetic activity, Diabetes mellitus. International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.64

How to cite this article: Abarnadevika A, Ariharasivakumar G, Anusha D, Nagalakshmi S. Development and Characterization of Ethanolic Extract of Cleome gynandra L. Loaded Solid Lipid Nanoparticles for the Management of Diabetes Mellitus. International Journal of Drug Delivery Technology. 2024;14(3):1704-1707.

REFERENCES

  1. Britannica, The Editors of Encyclopaedia. “diabetes mellitus”. Encyclopedia Britannica, 21 Jun. 2024, https://www.britannica.com/science/diabetes-mellitus. Accessed 23 June 2024.
  2. Aljahani, O., Khan, S., Jabal, F., Alhomoud. (2020). Herbal medicine from the perspective of type II diabetic patients and physicians: what is the relationship?. BMC Complementary Medicine and Therapies, 20 (1) 1-9. DOI:10.1186/s12906-020-2854-4
  3. Pham, B., Pham, L. Le. (2020). Bioactive compounds in antidiabetic plants: From herbal medicine to modern drug discovery. Biology. 9 (9) 252. DOI:10.3390/biology9090252
  4. Ekor. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4 177. DOI:10.3389/fphar.2013.00177
  5. P. Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zheng, H.B. Li. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18 (1) 96. DOI: 10.3390/ijms18010096
  6. Lakhssassi, N., Baharlouei, A., Watson, D.G., Watson, D.A. Lightfoot. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 6 (4) 42. doi: 10.3390/plants6040042
  7. V. Manyevere, A., Manyevere, E. Chakauya. (2022). Cleome gynandra: A wonder climate-smart plant for nutritional security for millions in semi arid areas. Frontiers in Plant Science, 13 1003080. DOI: 10.3389/fpls.2022.1003080
  8. Spanidi, E., Mourtzinos, I., Mourtzinos, K. Gardikis. (2021). Innovative delivery systems loaded with plant bioactive ingredients: Formulation approaches and applications. Plants. 10(6) 1238. DOI: 10.3390/plants10061238
  9. H. Islam, F., Islam, M. Sameem. (2012). Influence of nanotechnology on herbal drugs: A Review. Journal of advanced pharmaceutical technology & Research. 3 (3) 142.DOI:10.4103/2231-4040.101006
  10. S. Rahman, H.H. Othman, N.I. Hammadi, S.K. Yeap, K.M.Amin, N. Abdul Samad,
  11. Singh C, Yashwant, Gupta AK, Garg Formulation Development and Evaluation of Divalproex Sodium Extended-release Tablets. International Journal of Drug Delivery Technology. 2022;12(4):1769-1773.DOI: 10.25258/ijddt.12.4.46