International Journal of Drug Delivery Technology
Volume 14, Issue 3

Investigation of Olive Leaf Polyphenol Extract Loaded Silver Nanoparticle Optimization by Box-Behnken Design

Renganathan A*, Srinivasan R

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bharath Institute of Higher Education and Research, Chennai, Tamil Nadu, India

Received: 12th March, 2024; Revised: 03rd May, 2024; Accepted: 22nd August, 2024; Available Online: 25th September, 2024

ABSTRACT

Silver nanoparticles (AgNPs) are synthesized and optimized by reducing and stabilizing them with olive leaf extract. The successful synthesis of AgNPs was verified by UV-vis spectroscopy, SEM imaging, and DLS measurements, demonstrating characteristic features such as a surface plasmon resonance peak, spherical morphology, crystalline structure, and narrow size distribution with good stability. Utilizing Box-Behnken Design (BBD), the optimal synthesis conditions were determined, with a silver nitrate concentration of 1 mM, olive leaf extract volume of 10 mL, and reaction time of 60 minutes, yielding AgNPs with desirable properties. Statistical analysis revealed the significant influence of individual variables and their interactions on AgNP characteristics. Lower concentrations of silver nitrate and olive leaf extract led to smaller particle sizes and higher zeta potentials, while longer reaction times resulted in larger particles and lower zeta potentials. Among the experimental runs, Run 11 exhibited the most favorable properties, with 54 nm particle size, -36 mV zeta potential, and an encapsulation efficiency of 92.28%. ANOVA analysis further elucidated the significant effects of synthesis parameters on zeta potential, particle size, and encapsulation efficiency. In conclusion, the optimized formulation obtained through BBD offers a tailored approach for the synthesis of AgNPs with desired properties, suitable for various applications such as biomedical, catalytic, and sensing applications. The comprehensive understanding of synthesis parameters and their effects provided by this study facilitates the rational design of AgNPs for specific applications, enhancing their potential in diverse fields.

Keywords: Olive leaf extract, Polyphenols, Silver nanoparticles, Box-Behnken design, Optimization, Nanotechnology. International Journal of Drug Delivery Technology (2024); DOI: 10.25258/ijddt.14.3.09

How to cite this article: Renganathan A, Srinivasan R. Investigation of Olive Leaf Polyphenol Extract Loaded Silver Nanoparticle Optimization by Box-Behnken Design. International Journal of Drug Delivery Technology. 2024;14(3):1312-1322.

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