International Journal of Drug Delivery Technology
Volume 14, Issue 4

Anticancer Effect on MCF-7 Breast Cancer

Jawale J1*, Kashikar V1,2, Dhole S1, Devne S1

1Department of Pharmaceutics, PES Modern College of Pharmacy (For Ladies), Moshi, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India.

2Department of Pharmaceutics, Director, Amepurva Forum, Maharashtra, India. 

Received: 28th Aug, 2024; Revised: 22nd Oct, 2024; Accepted: 2nd Nov, 2024; Available Online: 25th Dec, 2024

ABSTRACT

In order to increase Taxifolin's solubility and bioavailability, this study set out to create and assess niosomes of Taxifolin powder by the thin film hydration approach. A nonionic surfactant called span 60 and a membrane stabiliser called cholesterol were utilised. Particle size distribution, zeta potential, and light scattering analysis were all examined. Niosomes demonstrated a mean particle size between 106.5± 7.42 nm and 115.8 ±11.61 nm, with particle size increasing as the surfactant and cholesterol molar ratios were raised. The percentage yield was found to be in range 44 ± 0.235% to 81 ± 0.216%. The optimized batch (FC6) had zeta potential of -27.7 ± 2.37 mV and 92.78 ± 3.14 %entrapment efficiency. Taxifolin loaded niosomes demonstrated sustained release of Taxifolin.The anticancer potential of compound was evaluated using an in vitro cytotoxicity assay on MCF-7 cell lines. Finally, associated to free Taxifolin, cytotoxic impact of Taxifolin loaded niosomes on MCF-7 cell line was found to be substantially improved by the MTT assay. Result demonstrated that solubility and bioavailability of naturally derived Taxifolin phytochemicals can be increase by loading it into niosomes, thus enhance their anticancer efficacy.

Keywords: Taxifolin, Phytochemical, Niosomes, MCF-7 Breast Cancer Cell.

How to cite this article: Jawale J, Kashikar V, Dhole S, Devne S. Development & Formulation of Taxifolin Loaded Niosomes for its Anticancer Effect on MCF-7 Breast Cancer. International Journal of Drug Delivery Technology.

2024;14(4):2040-47. doi: 10.25258/ijddt.14.4.14

REFERENCES

  1. Bothiraja C, Kapare HS, Pawar AP, Shaikh KS. Development of plumbagin-loaded phospholipid– Tween® 80 mixed micelles: formulation, optimization, effect on breast cancer cells and human blood/serum compatibility testing. Therapeutic delivery. 2013;4(10):1247-1259.https://doi.org/10.4155/tde.13.92
  2. Barani M, Mirzaei M, Torkzadeh-Mahani M, Nematollahi MH. Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer. DARU Journal of Pharmaceutical Sciences. 2018;26:11-17.
  3. Nicolaou KC, Yang Z, Liu JJ, Ueno H, Nantermet PG, Guy RK, Claiborne CF, Renaud J, Couladouros EA, Paulvannan K, Sorensen EJ. Total synthesis of taxol. Nature. 1994; 17;367(6464):630-634.
  4. Castro FA, Mariani D, Panek AD, Eleutherio EC, Pereira MD. Cytotoxicity mechanism of two naphthoquinones (menadione and plumbagin) in Saccharomyces cerevisiae. PloS one. 2008 22;3(12):e3999. DOI:10.1371/journal.pone.0003999
  5. Jin SE, Jin HE, Hong SS. Targeted delivery system of nanobiomaterials in anticancer therapy: from cells to clinics. BioMed research international. 2014;2014(1):814http://dx.doi.org/10.1155/2014/814208
  6. Sakpakdeejaroen I, Somani S, Laskar P, Mullin M, Dufès C. Transferrin‐bearing liposomes entrapping plumbagin for targeted cancer therapy. Journal of interdisciplinary nanomedicine. 2019;4(2):54-71. doi: 1002/jin2.56.
  7. Ahmad R, Srivastava S, Ghosh S, Khare SK. Phytochemical delivery through nanocarriers: A Colloids and Surfaces B: Biointerfaces. 2021; 1;197:111389.doi:https://doi.org/10.1016/j.colsurfb.2020.111389
  8. Jeetah R, Bhaw-Luximon A, Jhurry D. Nanopharmaceutics: phytochemical-based controlled or sustained drug-delivery systems for cancer treatment. Journal of biomedical nanotechnology. 2014;1;10(9):1810-1840.https://doi.org/10.1166/jbn.2014.1884.
  9. Chen X, Gu N, Xue C, Li BR. Plant flavonoid taxifolin inhibits the growth, migration and invasion of human osteosarcoma cells. Molecular medicine reports. 2018;17(2):3239-3245. doi: 10.3892/mmr.2017.8271.
  10. Kim YJ, Choi SE, Lee MW, Lee CS. Taxifolin glycoside inhibits dendritic cell responses stimulated by lipopolysaccharide and lipoteichoic acid. Journal of Pharmacy and Pharmacology. 2008;60(11):1465- 1472., https://doi.org/10.1211/jpp.60.11.0007
  11. Kang MJ, Eum JY, Park SH, Kang MH, Park KH, Choi SE, Lee MW, Kang KH, Oh CH, Choi YW. Pep-1 peptide-conjugated elastic liposomal formulation of taxifolin glycoside for the treatment of atopic dermatitis in NC/Nga International journal of pharmaceutics. 2010; 15;402(1-2):198-204. doi:10.1016/j.ijpharm.2010.09.030.
  12. Zu Y, Wu W, Zhao X, Li Y, Wang W, Zhong C, Zhang Y, Zhao X. Enhancement of solubility, antioxidant ability and bioavailability of taxifolin nanoparticles by liquid antisolvent precipitation technique. International journal of pharmaceutics. 2014;25;471(1-2):366- 376.doi: 10.1016/j.ijpharm.2014.05.049.
  13. Shikov AN, Pozharitskaya ON, Miroshnyk I, Mirza S, Urakova IN, Hirsjärvi S, Makarov VG, Heinämäki J, Yliruusi J, Hiltunen R. Nanodispersions of taxifolin: Impact of solid-state properties on dissolution International Journal of Pharmaceutics. 2009;30;377(1- 2):148-152.doi: 10.1016/j.ijpharm.2009.04.044.
  14. Rasenack N, Müller BW. Micron‐size drug particles: common and novel micronization techniques. Pharmaceutical development and technology. 2004;1;9(1):1-3. doi: 10.1081/pdt-120027417.
  15. Singh B, Sharma P, Bose S, Thakur J, Saini G, Vyas M. Niosomes: The current and future perspectives in drug delivery Research Journal of Pharmacy and Technology. 2021;14(7):3961- 3966.http://dx.doi.org/10.52711/0974- 360X.2021.00687
  16. Kumar P, Verma N. An overview on niosomes: As an auspesious drug delivery system on the bases of application. Research Journal of Pharmacy and 2021; 14(5):2896-2902.http://dx.doi.org/10.52711/0974-360X.2021.00508
  17. Avinash V, Umashankar MS,     Damodharan    Niosomes: A more promising tool to load poorly penetrating drug through skin for the treatment of Acne vulgaris.                Research        Journal ofPharmacy    and Technology. 2020;13(6):3035- 3040.http://dx.doi.org/10.5958/0974- 360X.2020.00536.3
  18. Hajizadeh MR, Maleki H, Barani M, Fahmidehkar MA, Mahmoodi M, Torkzadeh-Mahani M. In vitro cytotoxicity assay of D-limonene niosomes: an efficient nano-carrier for enhancing solubility of plant-extracted agents. Research in pharmaceutical sciences. 2019 Oct 1;14(5):448-458.doi: 10.4103/1735-5362.268206.
  19. Agarwal S, Mohamed MS, Raveendran S, Rochani AK, Maekawa T, Kumar DS. Formulation, characterization and evaluation of morusin loaded niosomes for potentiation of anticancer therapy. RSC advances. 2018;8(57):32621-32636.doi: 10.1039/c8ra06362a.
  20. Hajizadeh MR, Parvaz N, Barani M, Khoshdel A, Fahmidehkar MA, Mahmoodi M, Torkzadeh-MahaniDiosgenin-loaded niosome as an effective phytochemical nanocarrier: Physicochemical characterization, loading efficiency, and cytotoxicity assay. DARU Journal of Pharmaceutical Sciences. 2019;1;27:329-339.doi: 10.1007/s40199-019-00277-0.
  21. Dhole R, Patil U, Jadhav N. Stabilization of hydrochlorothiazide nanocrystals using fibroin. Marmara J. 2019;1;23:997- 1008.10.35333/jrp.2019.64.
  22. Joshi S, Sharma L, Tare M, Baheti D, Dama G, Tare The Nutritional Needs of Mothers and Babies: A Review. International Journal of Pharmaceutical Quality Assurance. 2023; 14(2):421-425.
  23. Kumar YA, Setty CM. Formulation and evaluation of maltodextrin based doxorubicin HCl proniosomes. Research Journal of Pharmacy and Technology. 2019;12(6):2776-278http://dx.doi.org/10.5958/0974-360X.2019.00466.9
  24. Olii AT, Nugroho AK, Martien R, Riyanto Effect of Ratio Span 60-Cholesterol on the Characteristic of Niosomes Vitamin D3. Research Journal of Pharmacy and Technology. 2022;15(12):5551-5554. http://dx.doi.org/10.52711/0974-360X.2022.00937
  25. Gaikwad DS, Chougale RD, Patil KS, Disouza JI, Hajare AA. Design, development, and evaluation of docetaxel-loaded niosomes for the treatment of breast cancer. Future Journal of Pharmaceutical Sciences. 2023;24;9(1):43.https://doi.org/10.1186/s43094-023-00494-0
  26. Khan DH, Bashir S, Khan MI, Figueiredo P, Santos HA, Peltonen L. Formulation optimization and in vitro characterization of rifampicin and ceftriaxone dual drug loaded niosomes with high energy probe sonication technique. Journal of Drug Delivery Science andTechnology.2020;1;  58:101763. https://doi.org/10.1016/j.jddst.2020.101763.
  27. Tyagi R, Waheed A, Kumar N, Ahad A, Bin Jardan YA, Mujeeb M, Kumar A, Naved T, Madan S. Formulation and Evaluation of plumbagin-loaded niosomes for an antidiabetic study: Optimization and in vitro Pharmaceuticals. 2023;17;16(8):1169.doi: 10.3390/ph16081169.
  28. Suriyaprakash Tn, Parthiban S, Prabu Sl, Sumathi A. Formulation and evaluation of paclitaxel niosome for its improved anti-cancer activity. ACTA Pharmaceutica Sciencia. 2011; 53(3).
  29. Mohanty C, Acharya S, Mohanty AK, Dilnawaz F, Sahoo SK. Curcumin-encapsulated MePEG/PCL diblock copolymeric micelles: a novel controlled delivery vehicle for cancer therapy. Nanomedicine. 2010;1;5(3):433-449.https://doi.org/10.2217/nnm.10.9
  30. Katrolia A, Chauhan SB, Shukla VK. Formulation and evaluation of metformin hydrochloride-loaded curcumin–lycopene niosomes. SN Applied Sciences. 2019;1:1-6.https://doi.org/10.1007/s42452-019-1768-6
  31. Negi P, Aggarwal M, Sharma G, Rathore C, Sharma G, Singh B, Katare OP. Niosome-based hydrogel of resveratrol for topical applications: An effective therapy for pain related disorder (s). Biomedicine & 2017;1;88:480-7. https://doi.org/10.1016/j.biopha.2017.01.083
  32. Graidist P, Martla M, Sukpondma Cytotoxic activity of Piper cubeba extract in breast cancer cell lines. Nutrients. 2015;10;7(4):2707-2718.https://doi.org/10.3390/nu7042707
  33. Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, Minor Cell viability assays. Assay guidance manual . 2016;1.
  34. Van Meerloo J, Kaspers GJ, Cloos J. Cell sensitivity assays: the MTT assay. Cancer cell culture: methods and 2011:237- 245.https://doi.org/10.1007/978-1-61779-080-5_20.
  35. Kumar S, Baldi A, Sharma DK. In vitro antioxidant assay guided ex vivo investigation of cytotoxic effect of phytosomes assimilating taxifolin rich fraction of Cedrus deodara bark extract on human breast cancer cell lines (MCF7). Journal of Drug Delivery Science and 2021;1;63:102486. 10.1016/j.jddst.2021.102486.
  36. Wang CF, Fan L, Tian M, Qi XS, Liu JX, Feng JB, Du SS, Su X, Wang YY. Radiosensitizing effect of schinifoline from Zanthoxylum schinifolium Sieb et Zucc on human non-small cell lung cancer A549 cells: A preliminary in vitro investigation. Molecules. 2014;1;19(12):20128-20138. doi:
  37. 10.3390/molecules191220128.
  38. Ertus EB, Gulbahce-Mutlu E, Alpa S, Ozturk A. Production and biological properties of nano porous glass microparticles for anticancer drug Journal of Applied Pharmaceutical      Science. 2024;14.http://doi.org/10.7324/JAPS.2024.168308
  39. Beconi MG, Howland D, Park L, Lyons K, Giuliano J, Dominguez C, Munoz-Sanjuan I, Pacifici R. Pharmacokinetics of memantine in rats and PLoSCurrents.2011;15;3.https://doi.org/10.1371/currents.RRN1291
  40. Mane V, Killedar S, More H, Tare H. Preclinical study on camellia sinensis extract-loaded nanophytosomes for enhancement of memory-boosting activity: optimization by central composite design. Future Journal of Pharmaceutical Sciences. 2024;1;10(1):66.
  41. Ahmad S, Shaikh TJ, Patil J, Meher A, Chumbhale D, Tare H. Osmotic Release Oral Tablet Formulation, Development, and Evaluation of an Anti-epileptic International Journal of Drug Delivery Technology. 2023; 13(1):305-312.
  42. Ahmad S, Mujawar T, Batewal B, More P, Gaikwad A, Chumbhale D, Tare RP-UHPLC and HPTLC methoddevelopment and validation for analysis of andrographolide from herbal hepatoprotective formulation. International Journal of Pharmaceutical Quality Assurance. 2023; 14:96-104.
  43. Dragicevic N, Maibach HI. Liposomes and Other Nanocarriers for the Treatment of Acne Vulgaris: Improved Therapeutic Efficacy and Skin Tolerability. Pharmaceutics. 2024 Feb 22; 16(3):309.
  44. Mane V, Killedar S, More H, Nadaf S, Salunkhe S, TareNovel Phytosomal Formulation of Emblica officinalis Extracts with Its In Vivo Nootropic Potential in Rats: Optimization and Development by Box‐ Behnken Design. Journal  of  Chemistry.  2024;(1):6644815. https://doi.org/10.1155/2024/6644815.