The goal of the experiment was to prepare an antimicrobial tooth powder using the fruit juice of Punica granatum for the treatment of dental caries. Plain pomegranate fruit juice, spray-dried fruit juice, and freeze-dried fruit juice were compared for their antimicrobial activity against Streptococcus mutans and Candida albicans and further formulated into toothpowder.Punica granatum fruit juice was extracted sprayed and lyophilized. These powders were further transformed into toothpowder. Conversion of liquid juice to solid powder: Pomegranate juice and Neusilin US2 were mixed in a 1:1 weight ratio to obtain a free-flowing juice powder. Spray-dried powder formulation Maltodextrin and β-cyclodextrin were slowly added to deionized water (67%) and heated to 51°C and the resulting solution was obtained. The obtained solution is cooled at room temperature. Pomegranate fruit juice (33%) was added to solution, stirred for 1 hour, and transferred to a spray dryer. Freezing of pomegranate juice Fresh pomegranate juice (20 ml) was filtered and frozen at 2-3 ºC. The cold juice was transferred to a freezer with a 15-minute heating time. After complete drying (20-24 h), Neusilin US2 (3 g) was added as an adsorbent to remove the stickiness of the freeze-dried powder from the powder mass.Pomegranate juice sprayed and freeze-dried fruit juice should have good antimicrobial activity. Based on the obtained data, it can be concluded that 7.5% spray-dried tooth powder and freeze-dried tooth powder have excellent antimicrobial activity in tooth powder...
Keywords: Pomegranate, antioxidant, tooth powder, anti-fungal, anti-microbial.
How to cite this article: Patil S,Kamble S,Salvi A,Kaygude U, Antimicrobial Tooth Powder Formulated from Punica Granatum Fruit Juice: Comparative Analysis of Plain, Spray-Dried, And Freeze-Dried Variants Against Streptococcus Mutans and Candida Albicans. Int J Drug Deliv Technol. 2025;15(4): 1701-1711, DOI: 10.25258/ijddt.15.4.21