Objective: The study was aimed at exploring the development of polyherbal formulation of aloe vera as Kumaryasava using statistical approach and evaluates therein vitro antioxidant, in vivo hepatoprotective activity. Method: The Kumaryasava formulation was statistically optimized using Box-Behenken Design (BBD). The effect of independent variables sweetening agent, fermentation duration and fermentation temperature was analyzed on response alcohol content (%) generated determined by gas chromatography. Further, the formulation was investigated for its physicochemical parameters, in vitro and in vivo studies. Result:In vitro antioxidantscreening was performed by DDPH scavenging assay designated that the polyherbal formulation has 82.70 ± 0.98 % potential to deactivate free radicals than standard ascorbic acid.Liver enzyme levels including glutathione, catalase, and superoxide dismutase were shown to be enhanced in wistar rats that underwent in vivo hepatoprotective activity in response to CCl4-induced liver damage. Additionally, the histological examinations showed that the modified formulation significantly improved liver tissues compared to the standard. Conclusion:Hence, the Kumaryasava formulation being an effective antioxidant that indicates elevating effect on hepatic enzyme and has potential in managing liver disorders.
Keywords: Kumaryasava, Madhu, Antioxidant, Aloevera, Hepatoprotective, Polyphenols
How to cite this article: Gaikwad P; Patil SS, Statistical Optimization Applying Box Behenkent Design to Kumaryasava Formulation for it’s In Vivo Antioxidant and Ccl4 Induced Hepatoprotective Activity. Int J Drug Deliv Technol. 2025;15(4): 1762-1771, DOI: 10.25258/ijddt.15.4.27