The research was focused on assessing the pharmacological efficacy of protein-loaded nanoparticles against dinitrosoethylamine and carbon tetrachloride-induced hepatocellular carcinoma. Four individual groups of forty eight swiss-albino male mice were designed and induced with a single dose of dinitrosethylamine at 200 mg/kg body weight two weeks before administration of a 2 mL/kg dosage of carbon tetrachloride administered once a week along with 5-Flourouracil at 10 mg/kg, b.wt., two times a week for 12 weeks. Plasma, tissue, inflammatory, and cancer-specific indicators were assessed for anti-cancer efficacy of protein-loaded nanoparticles in comparison to 5-fluorouracil (5-FU). Histological analysis was conducted to examine tissue architecture and validate the results of the study. Lactoferrin-loaded solid lipid nanoparticles (LSLN) have demonstrated a significant ameliorative effect in restoring abnormal levels of plasma, tissue, inflammatory, and cancer-specific markers, as evidenced by histopathological examinations compared to group II hepatoma and group III 5-FU. The study has demonstrated that primary dietary proteins designed nanoparticles inhibit tumor development by enhancing the antioxidant system's capacity to reduce cancer cells..
Keywords: Hepatocellular Carcinoma, Dinitrosoethylamine, Carbon Tetrachloride, Lactoferrin Nanoparticles, Oxidative Stress.
How to cite this article: Gandla M; Mudduluru NB, Pharmacological Assessment of Protein Loaded Nanoparticles for Anticancer Activity. Int J Drug Deliv Technol. 2025;15(4): 1805-1813, DOI: 10.25258/ijddt.15.4.33