The Role of Eurycoma longifolia (Pasak Bumi) in Modulating the Apoptosis Pathway in Diffuse Large B-Cell Lymphoma (DLBCL): In silico study on JAK-STAT Pathways

Sigit P. Kurniawan1, Ika K. Oktaviyanti2, Roselina Panghiyangani3*, M. Darwin Prenggono4 and Huldani5

1Doctoral Student, Medical Sciences Program, Faculty of Medicine and Health Sciences, Lambung Mangkurat University/
1Department of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Lambung Mangkurat University/Ulin General Hospital, Banjarmasin, Indonesia
2Department of Pathology Anatomy, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia
3*Department of Biomedicine, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia
4Department of Hematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine, Lambung Mangkurat University/Ulin General Hospital, Banjarmasin, Indonesia
5Department of Physiology, Faculty of Medicine, Lambung Mangkurat University, Banjarmasin, Indonesia
* rpanghiyangani@ulm.ac.id

Received: 14th Sep, 2025; Revised: 28th Oct 2025; Accepted: 6th Nov, 2025; Available Online: 1st December, 2025

ABSTRACT

Diffuse Large B-Cell Lymphoma (DLBCL) is an aggressive type of non-Hodgkin's lymphoma and has a poor prognosis, but still has the potential to be cured with combination chemotherapy, but has a high rate of resistance and recurrence. Resistance to apoptosis is the main reason for the occurrence of chemoresistance in DLBCL is associated with the inhibition of the modulation of the apoptotic signaling pathway. Pasak bumi (Eurycoma longifolia) is one of the native Indonesian plants which has anti-cancer potential by inducing the apoptosis pathway mechanism, with one of the contents of pasak bumi root, namely eurycomanol which has been proven to be cytotoxic in solid tumors. Protein tracking was carried out using the UniProt database (https://www.uniprot.org/), with the bioactive components of pasak bumi and STAT-3 as target proteins. Molecular docking was carried out using Patchdock Server and antioxidant activity (https://services.healthtech.dtu.dk/service.php?AnOxPePred-1.0) to determine affinity and interactions with target proteins in cancer cells. Researchers found that the peptide (quassionoid/flavonoid) eurycomanol content in pasak bumi has the potential to modulate apoptosis pathway in its role as an anti-cancer through stimulation of pro-apoptotic genes and inhibition of anti-apoptotic genes by activating JAK-STAT Pathways.

Keywords: DLBCL - JAK-STAT pathway - Apoptosis - Eurycoma longifolia

How to cite this article: Kurniawan SP, Oktaviyanti IK, Panghiyangani R, Prenggono MD, Huldani H; The Role of Eurycoma longifolia (Pasak Bumi) in Modulating the Apoptosis Pathway in Diffuse Large B-Cell Lymphoma (DLBCL): In silico study on JAK-STAT Pathways. Int J Drug Deliv Technol. 2026;16(1): 421-428. DOI: 10.25258/ijddt.16.1.44