1*Research scholar, Karpagam Academy of Higher Education, Coimbatore. Email Id: saranyabalank12@gmail.com
2Professor, HOD Pharmaceutics, Karpagam Academy of Higher Education, Coimbatore. Email Id: drsaravanan.g@kahedu.edu.in
Objective: The present study aimed to evaluate the phytochemical composition of Alstonia venenata (R. Br.) leaf extracts using gas chromatography–mass spectrometry (GC–MS) and to assess their in vitro antioxidant activity.
Methods: Leaves of Alstonia venenata were sequentially extracted using hexane, ethyl acetate, ethanol, and water to obtain solvent fractions of varying polarity. The extracts were analyzed by GC–MS to identify volatile and semi-volatile phytochemicals. Antioxidant activity was evaluated using DPPH radical scavenging and hydrogen peroxide (H₂O₂) scavenging assays, and IC₅₀ values were determined.
Results: GC–MS analysis revealed the presence of several phytochemical classes including terpenoids, phenolic compounds, fatty acid derivatives, and alkaloids. Among the tested extracts, the ethanolic fraction exhibited the strongest antioxidant activity with IC₅₀ values of 19.97 μg/mL for DPPH and 2.27 μg/mL for the H₂O₂ assay.
Conclusion: The findings indicate that A. venenata leaves contain diverse phytochemical constituents and demonstrate significant in vitro antioxidant activity, particularly in the ethanolic extract. Further studies are required to isolate and characterize the active compounds responsible for the observed antioxidant activity.
KEYWORDS: Alstonia venenata, GC–MS analysis, Phytochemical profiling, Antioxidant activity, DPPH scavenging assay
How to cite this article: Balan S, Saravanan G. Analytical Evaluation of Antioxidant Activity Through GC-MS Profiling: A Comparative Study of Phytochemical Constituents in Alstonia Venenata Leaf Extract. Int J Drug Deliv Technol. 2026;16(10s): 375-386; DOI: 10.25258/ijddt.16.10s.49
Source of support: Nil.
Conflict of interest: None