1,2Chettinad School of Pharmaceutical Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam-603103, Tamilnadu, India & Department of Pharmacology, St. John's College of Pharmaceutical Sciences and Research, Kattappana-685508, Kerala, India. Email: ambilyscaria.ms@gmail.com. ORCID: 0000-0002-9960-2954
1*Department of Pharmaceutical Sciences, Chettinad School of Pharmaceutical Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam-603103, Tamilnadu, India. Email: elphineprabahara@care.edu.in. ORCID: 0000-0001-5401-0149. Mobile No: +91 7702976939
3Department of Pharmacology, College of Pharmaceutical Sciences, Pariyaram Medical College, Kannur-670503, Kerala, India. Email: sijuellickal@rediffmail.com. ORCID: 0000-0002-4161-2770
4Chettinad School of Pharmaceutical Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam-603103, Tamilnadu, India. Email: lakshmicsps@gmail.com. ORCID: 0000-0002-2460-0073
Zephyranthes rosea, member of the family Amaryllidaceae, contains a rich source of several neuroactive alkaloids, including Galantamine, with established relevance in Alzheimer's disease management. While the bulb constituents have been investigated previously, the biological potential of the leaves remain largely unexplored.
The present study aimed to characterize the phytochemical composition of ethanolic extract of Z. rosea leaves (ZRLE) by GC–MS and to evaluate its antioxidant and acetylcholinesterase inhibitory activities, along with its cytotoxicity and neuroprotective efficacy against aluminium-induced neurotoxicity in SH-SY5Y cells.
GC–MS identified 33 phytochemicals in ZRLE, including galantamine-related alkaloids. The extract demonstrated notable antioxidant activity as well as moderate AChE inhibition. Cytotoxicity studies showed an LC₅₀ of 85.38 µg/mL in SH-SY5Y cells. Aluminium chloride markedly reduced cell viability, while extract pre-treatment significantly attenuated neurotoxicity and restored cell viability with observable morphological protection. These findings suggest that ZRLE exerts significant neuroprotective activity.
Keywords: Zephyranthes rosea; GC–MS; anti-oxidant; neuroprotection; aluminium chloride; SH-SY5Y cells; MTT assay
How to cite this article: Scaria A, Prabahar AE, Siju EN, Lakshmi K. Phytochemical Profiling and Neuroprotective Potential of Zephyranthes rosea Leaf Extract Against Aluminium-Induced Neurotoxicity in SH-SY5Y Cells. Int J Drug Deliv Technol. 2026;16(10s): 421-430; DOI: 10.25258/ijddt.16.10s.53.
Source of support: Nil.
Conflict of interest: None