*Associate Professor, Department of Pharmaceutical Analysis, Santhiram College of Pharmacy (Autonomous), Nandyal-518501, A.P., India. Email: chintalaparimaladevi@gmail.com
1Professor, Department of Pharmaceutical Analysis, Santhiram College of Pharmacy (Autonomous), Nandyal-518501, A.P., India. Email: muneer.pharma@gmail.com
2Associate Professor, Department of Pharmaceutical Analysis, Santhiram College of Pharmacy (Autonomous), Nandyal-518501, A.P., India. Email: Maheswari.kukutla@gmail.com
3Associate Professor, Department of Pharmaceutical Analysis, Santhiram College of Pharmacy (Autonomous), Nandyal-518501, A.P., India. Email: ruthmanorma5@gmail.com
4Department of Pharmaceutical Analysis, Santhiram College of Pharmacy (Autonomous), Nandyal-518501, A.P., India. Emails: busaganideepika576@gmail.com, katikazakeer789@gmail.com, vineelajessica2003@gmail.com, moghalmubeenakousar@gmail.com
A robust and economical UV spectrophotometric method was established and systematically validated for the quantitative determination of brimonidine tartrate in bulk drug and pharmaceutical dosage forms. The analysis was performed using a suitable solvent system, where brimonidine tartrate exhibited optimal absorbance at its characteristic wavelength. The method demonstrated linearity over a defined concentration range, conforming to Beer–Lambert's law with a high correlation coefficient. Validation of the proposed method was carried out in compliance with ICH guidelines. Method accuracy was verified through recovery studies, while precision was assessed by repeatability and intermediate precision experiments. The validated method exhibited satisfactory sensitivity, reproducibility, and robustness. Application of the method to a marketed pharmaceutical formulation confirmed its suitability for routine quality control, as no interference from formulation excipients was observed. Owing to its simplicity, rapidity, and cost-effectiveness, the proposed UV spectrophotometric method is well suited for regular analytical evaluation of brimonidine tartrate in quality assurance laboratories.
Keywords: Brimonidine tartrate assay, UV–visible spectroscopy, Analytical method optimization, Regulatory validation, pharmaceutical formulation analysis, Quality assurance testing.
How to cite this article: Devi CP, Muneer S, Maheswari K, Manorama M, Deepika B, Zakeer K, Vineela N, Kousar MM, Aswini G. Quality-By-Design Assisted UV Spectrophotometric Method for Estimation of Brimonidine Tartrate in Bulk and Pharmaceutical Dosage Forms. Int J Drug Deliv Technol. 2026;16(10s): 582-586; DOI: 10.25258/ijddt.16.10s.70
Source of support: Nil.
Conflict of interest: None