1*Research Scholar, Department of Pharmacy, Madhav University, Pindwara, Sirohi, Rajasthan. Mail Id: surajb905@gmail.com
2Professor and Principal, Institute of Pharmacy, Madhav University, Pindwara, Sirohi, Rajasthan, Pin-307032
The present study was undertaken to evaluate the physicochemical properties, phytochemical constituents, thin layer chromatographic (TLC) profile, and antioxidant potential of the hydroalcoholic extract of Scaphium affine seeds. Physicochemical parameters such as loss on drying, total ash, acid insoluble ash, and water-soluble ash were determined for the standardization of the crude drug. The powdered seeds were observed as dark brown, bitter, and pungent in nature with loss on drying of 4.3%, total ash value of 3.5%, acid insoluble ash of 1.06%, and water-soluble ash of 7.94%. Fluorescence analysis showed characteristic color changes under normal and ultraviolet light, which helps in the identification and authentication of the plant material. The hydroalcoholic extraction yielded 4.62% (w/w) extract. Preliminary phytochemical screening revealed the presence of flavonoids, phenolic compounds, and diterpenes, while other constituents such as alkaloids, glycosides, carbohydrates, proteins, saponins, tannins, and steroids were absent. TLC profiling confirmed the presence of phenolic and flavonoid compounds, showing characteristic Rf values comparable with standard compounds such as quercetin and gallic acid. The antioxidant activity of the extract was evaluated using DPPH radical scavenging and hydrogen peroxide scavenging assays. The hydroalcoholic extract exhibited concentration-dependent antioxidant activity with IC₅₀ values of 97.47 µg/ml in the DPPH assay and 36.42 µg/ml in the hydrogen peroxide scavenging assay. Although the activity was lower than the standard ascorbic acid, the results indicate that the extract possesses moderate antioxidant potential due to the presence of phenolic and flavonoid constituents. These findings suggest that Scaphium affine seeds may serve as a potential natural source of antioxidant compounds for therapeutic applications.
Keywords: Scaphium affine, Hydroalcoholic extract, Phytochemical screening, Thin layer chromatography, Antioxidant activity, DPPH assay, Hydrogen peroxide scavenging, Phenolic compounds.
How to cite this article: Bhagat SD, Dwivedi AK. Phytochemical Screening, TLC Profiling and Antioxidant Activity of Hydroalcoholic Extract of Scaphium Affine Seeds. Int J Drug Deliv Technol. 2026;16(10s): 683-691; DOI: 10.25258/ijddt.16.10s.82
Source of support: Nil.
Conflict of interest: None