1Visiting Senior Lecturer, Department of Botany, Govt. Homescience PG Lead College, Narmadapuram (MP), Pin Code – 461001
2Assistant Professor, M.S Ramaiah College of Arts, Science and Commerce, MSRIT post, Mathikere, Bangalore-54
3Assistant Professor, Department of Botany, Dr. B. R. Ambedkar Open University, Hyderabad, Telangana – 500033, India
4*Assistant Professor, Department of Botany, Shri Sadguru Gangageer Maharaj Science, Gautam Arts & Sanjivani Commerce College, Kopargaon, Dist. Ahmednagar, Maharashtra, India. Email: namdeoadmuthe@gmail.com
The quick development which created multidrug-resistant (MDR) infections all over the world has created an urgent need for new biocompatible antimicrobial drugs. The study created silver nanoparticles (AgNPs) through a green method which used aqueous leaf extracts from Azadirachta indica (Neem) and Ocimum sanctum (Tulsi) and Aloe vera. The successful bioreduction of silver ions was validated by UV–Visible spectroscopy which displayed characteristic surface plasmon resonance (SPR) peaks between 425 and 445 nanometer. The research used Transmission Electron Microscopy (TEM) to show that O. sanctum-generated AgNPs produced mostly spherical nanoparticles which had the smallest average particle size of approximately 18 nanometers. The study applied Fourier Transform Infrared (FTIR) spectroscopy to detect polyphenols and protein compounds which functioned as the primary materials that prevented nanoparticles from deteriorating.
The researchers evaluated the antibacterial effectiveness of AgNPs against clinical isolates of Escherichia coli and Pseudomonas aeruginosa through agar well diffusion and micro-broth dilution testing. Of the three formulations, O. sanctum-derived AgNPs had the highest antibacterial efficacy, with a minimum inhibitory concentration (MIC) of 6.25 µg/mL. The growth kinetic tests proved that the AgNPs operate as a bactericidal agent through two main processes which include membrane rupture and oxidative stress that reactive oxygen species create.
The research results demonstrate that O. sanctum phytosynthesized AgNPs function as a sustainable and powerful biological solution which can replace conventional antibiotics to treat resistant Gram-negative infections. The research demonstrates how traditional medicinal herbs can work together with nanotechnology to produce new advanced antimicrobial treatments.
Keywords: Azadirachta indica, Ocimum sanctum, Surface Plasmon Resonance, Minimum Inhibitory Concentration, Advanced Antimicrobial Treatments etc.
How to cite this article: Shrivastava R, Kumari HG, Ramakrishna N, Admuthe N. Comparative Evaluation of Phytosynthesized Silver Nanoparticles from Medicinal Plants Against Clinical Isolates of E. coli and Pseudomonas aeruginosa. Int J Drug Deliv Technol. 2026;16(11s): 1-8; DOI: 10.25258/ijddt.16.11s.1
Source of support: Nil.
Conflict of interest: None