1Associate Professor, Eklavya College of Pharmacy Tasgaon. Email: chitrarajput1234@gmail.com; ORCID: 0000-0002-4634-0022
2Principal, Dr. Ashok Gujar Institute of Pharmacy, Karad. Email: sachinpatilkrd@gmail.com; ORCID: 0000-0001-5162-3089
3Bharati Vidyapeeth College of Pharmacy, Belapur Navi Mumbai. Email: pallaviapatil2007@gmail.com; ORCID: 0009-0003-7217-128X
*Corresponding Author: Chitra Govind Rajput, Associate Professor, Eklavya College of Pharmacy Tasgaon. Email: chitrarajput1234@gmail.com
The neurological condition known as epilepsy is defined by convulsive seizures. 50–70 million People all throughout the world are affected and safer drugs with better anticonvulsant properties and more Accessibility is still required. The adverse effects of antiepileptic drugs remain a major issue not withstanding developments in pharmacotherapy, since they may reduce adherence and quality of life. Herbal remedies are becoming increasingly well-liked as alternative and complementary medicines as a result. The goal of this research was to investigate albino mice for the antiepileptic properties of the leaf extract Rumex acetosa L. in methanol.
Method: The Soxhlet extraction method was used to extract the leaves of Rumex acetosa L. using methanol. Testing for phytochemicals was done on the extract. The chemical components were separated using TLC and column chromatography. FTIR and GCMS-MS were used to examine fractions. The antiepileptic and acute oral toxicity of methanolic extract were evaluated. An intraperitoneal dose of 35 mg/kg PTZ in saline solution was used to induce seizures in order to test for antiepileptic activity. Swiss albino mice were given dosages of 200 and 400 mg/kg body weight of the methanolic extract to assess its antiepileptic properties using the PTZ procedure. Diazepam, at a dosage of 2 mg/kg, is the usual medication utilised. Microscopy-based histopathological analysis of brain tissue samples to look for cellular abnormalities, inflammation, or other pathological changes that could be linked to antiepileptic effects.
Result: Alkaloids, steroids, glycosides, amino acid flavonoids, carbohydrates, and phenolic tannins are among the phytochemicals found in methanolic extracts of the leaves of the Rumex acetosa L. plant. Yields 8 % in methanol extract; FTIR and GCMS-MS tests show different chemicals. At 200 and 400 mg/kg body weight, Rumex acetosa L extract produced p-values < 0.05 for epileptic score, p < 0.001 for convulsion latency, and p < 0.001 for convulsion duration. The protection value as a percentage was p < 0.001. Furthermore, the extract has dosage-dependent action, meaning that its activity rises with increasing dose. According to a brain histopathology investigation, there is some neuroprotective action.
Conclusion: Rumex acetosa L plant leaf methanolic extracts shows significant Antiepileptic activity.
Key Words: Rumex acetosa, Antiepileptic activity, Histopathology, Caryophyllene, GABA, CB2 receptors, PTZ Model
How to cite this article: Rajput CG, Patil SV, Patil PA. Evaluation Antiepileptic Activity of Rumex Acetosa L and Histopathological Changes in Mice Brain. Int J Drug Deliv Technol. 2026;16(11s): 244-253; DOI: 10.25258/ijddt.16.11s.23
Source of support: Nil.
Conflict of interest: Nil