International Journal Of Drug Delivery Technology
Volume 16, Issue 11s, 2026 | PG 700-708 | Article No 71

Development And Evaluation Of Eulophia Nuda Root Extract-Loaded Transdermal Drug Delivery System For Enhanced Anticancer Activity

Kiran Baviskar1*, Kalluri Satish Kumar2, Bhagyashri Ahirrao3, Gurmeet Singh Chhabra4, Almas Pathan3, Dhruvi Patel5, Rekha Devi Allagadda6, Rakesh Dhole3

1Associate Professor, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, India - 425107. Email: 22kar.kiran@gmail.com

2Professor, Y.D. Mane Institute Of Pharmacy, Kagal, Kolhapur, India - 416216.

3Assistant Professor, KVPS Maharai Ahilyabai Holkar College of Pharmacy, Shirpur, India - 425405.

4Professor, Indore Institute of Pharmacy, Pithampur Road, Opposite Indian Institute of Management Rau, Indore, India - 453331.

5Nutritional Biochemistry, Navrachana University, Vasna - Bhayli Road, Vadodara, Gujarat, India - 391410.

6Professor, Seven Hills College of Pharmacy, Autonomous, Venkatramapuram, Tirupati, India - 517561.

*Author for Correspondence: Kiran Baviskar, Associate Professor, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, India - 425107. Email: 22kar.kiran@gmail.com


ABSTRACT

This study describes the development and evaluation of a transdermal drug delivery system (TDDS) incorporating ethanolic extract of Eulophia nuda roots for potential anticancer activity. The optimized formulation (F5) exhibited uniform. The optimized formulation (F5) exhibited uniform and smooth morphology (thickness 0.32±0.03 mm, weight 185±5.2 mg), had high drug content (95.8±2.1%), folding endurance (>245 folds) and released uniformly (78.5±3.2% in 24 hours). FTIR and DSC were found to be compatible and SEM showed smooth, bead-free structures. Ex-vivo permeation experiments revealed successful transdermal delivery with a steady state flux of 12.5±1.0 µg/cm²/h and permeability coefficient of 6.25x10⁻³ cm/h. In-vitro MTT assay revealed dose-dependent cytotoxic activity against MCF-7, HeLa, A549 and HepG2 cell lines. The value of IC50 decreased (p < 0.001) 45.2-65.8 µg/mL TDDS formulation vs. 125.5-185.2 µg/mL crude extract. The IC₅₀ values of the optimized TDDS formulation were significantly lower than those of the crude extract, indicating enhanced anticancer activity. Taken together, these findings indicate that Eulophia nuda-based TDDS has the capacity to deliver safe, effective and controlled anticancer treatment with increased patient compliance and reduced systemic toxicity.

Keywords: Transdermal drug delivery, Eulophia nuda, HPMC, Anticancer activity, MTT assay, Sustained release, Phytochemicals

How to cite this article: Baviskar K, Kumar KS, Ahirrao B, Chhabra GS, Pathan A, Patel D, Allagadda RD, Dhole R. Development And Evaluation Of Eulophia Nuda Root Extract-Loaded Transdermal Drug Delivery System For Enhanced Anticancer Activity. Int J Drug Deliv Technol. 2026;16(11s): 700-708. DOI: 10.25258/ijddt.16.11s.71

Source of support: Nil.

Conflict of interest: None