1Department of Post Graduate Studies and Research in Microbiology, Jnana Sahyadri, Shankaraghatta, Shivamogga-577451, Karnataka, India
2Department of Post Graduate Studies and Research in Microbiology, Jnana Sahyadri, Shankaraghatta, Shivamogga-577451, Karnataka, India
3Niranthara Scientific Solutions Pvt. Ltd, #74, Hruday Arcade, Doddabele Main Road, Kengeri, Bengaluru-560 060
4Department of Post Graduate Studies and Research in Biochemistry, Kuvempu University, Jnana Sahyadri, Shankaraghatta, Shivamogga-577451, Karnataka, India
5Department of Post Graduate Studies and Research in Microbiology, Jnana Sahyadri, Shankaraghatta, Shivamogga-577451, Karnataka, India
Corresponding Author: N.B. Thippeswamy, Department of Post Graduate Studies and Research in Microbiology, Jnana Sahyadri, Shankaraghatta, Shivamogga-577451, Karnataka, India. Email: nbtmicro@gmail.com
The purpose of this research study is to examine the toxicological effects of Simarouba glauca DC., leaves (SGL) and stem bark (SGB) aqueous extract in mice as per the conventional acute oral toxicity test. In this study, the mice were administered a single dose of a leaf or stem bark aqueous extract at varying concentrations (1000 to 5000mg/kg b.w.). The mice were observed for body weight, general behavioral changes, adverse effects and mortality up to 14 days, post-treatment. In sub-acute toxicity studies, the leaf or stem bark aqueous extract was given orally to mice at varying doses of 100, 250, and 500mg/kg b.w for 28 days. The body weight, food and water intake were monitored throughout the study period and the relevant hematological, biochemical and histopathological evaluation was carried out at the end of the experiment. By acute toxicity study, the LD50 was found to be >5000mg/kg for both SGL and SGB groups. Likewise, in sub-acute toxicity studies of SGL and SGB-treated mice, the mean body weight was found to be significant (p<0.001). The hematological and biochemical evaluation of both extracts affirmed that the dose-dependent variations are not statistically significant. The histopathological findings suggest that SGL and SGB extracts are nontoxic to the liver, kidney and spleen. In conclusion, all these data suggest that the Simarouba glauca extracts are non-toxic and could be used as a safe herbal medicine against various infectious diseases and for the treatment of obesity.
Keywords: Simarouba glauca DC., Acute and sub-acute oral toxicity, Serum enzymes, Liver, Kidney, Spleen.
How to cite this article: Navya N, Veena H, Sandesh K, Achur RN, Thippeswamy NB. Assessment of acute and sub-acute oral toxicity of aqueous leaf and stem bark extract of Simarouba glauca DC. Int J Drug Deliv Technol. 2026;16(12s): 94-106. DOI: 10.25258/ijddt.16.12s.11
Source of support: Nil.
Conflict of interest: None