1Department of Microbiology, The University of Agriculture, Peshawar, Pakistan. Email: abida.n88r@gmail.com
2Department of Microbiology & Medical Laboratory Technology, Bahria University of Health Sciences, Karachi Campus, Pakistan. Email: naintara.bumdc@bahria.edu.pk
3Department of Biology, Urganch State University, Urgench, Uzbekistan. Email: xolisxonraximova744@gmail.com; ORCID: 0009-0001-2953-1900
4Department of Medical Fundamental Sciences, Termez University of Economics and Service, Termez, Uzbekistan. Email: zebiniso_alimova@tues.uz; ORCID: 0009-0003-5638-0786
5Department of Sport and Psychology, Mamun University, Khiva, Uzbekistan. Email: ruziyeva_m@mamunedu.uz; ORCID: 0009-0006-8541-1037
6Department of Natural Science, Academic Lyceum of Urgench Branch of the Tashkent Medical Academy, Urgench, Uzbekistan. Email: saodat_js@mail.ru; ORCID: 0009-0006-0173-6660
7*Department of Allied Health Sciences, Iqra National University, Peshawar, Pakistan. Email: farahshireen@inu.edu.pk
*Corresponding Author: Farah Shireen, Department of Allied Health Sciences, Iqra National University, Peshawar, Pakistan. Email: farahshireen@inu.edu.pk
Background: The Aspergillus species are the potent and promising bio-objects for the fabrication of nanoparticles. Among them, Aspergillus nidulans and Aspergillus terreus are the ideal biocatalysts for the synthesis of AgNPs. The AgNPs were prepared in laboratory of Allied Health Sciences Department, Sarhad University, Peshawar. Whereas, the nanoparticles were characterized in Centralized Resource Laboratory (CRL), University of Peshawar. The tube test was used to analyze the antifungal activity of nanoparticles against pathogenic fungi (Dermatophyte, Malassezia and Trichuris trichura).
Results - Synthesis and Characterization: A noticeable visual change in color from colorless to a dark brown confirmed the formation of AgNPs. The SEM characterization of A. terreus-derived AgNPs showed the presence of amorphous and distinctly asymmetrical particles. On the other hand, the SEM characterization of A. nidulans-derived AgNPs showed anisotropic shapes i.e., spherical and triangular morphologies. The XRD pattern of A. terreus-derived AgNPs exhibited a diffused pattern with no intense and clear peaks across the entire 2θ range from 5° to 80°, which showed that the AgNPs are amorphous.
Antifungal Activity: The synthesized AgNPs showed inhibitory activity against different fungal species including Malassezia (M-1), Malassezia (M-2), Dermatophyte (D-1), Dermatophyte (D2) and Trichuris trichiura (T-1) and Trichuris trichiura (T-2). The AgNPs synthesized using A. terreus specie showed highest inhibition rate (76.66%) against Trichuris trichiura (T-1). Whereas, less inhibitory effect was observed against Malassezia (M-1). The A. nidulans synthesized AgNPs also showed inhibitory activity against the tested fungal species. In this case, highest antifungal activity was observed against Malassezia (M-2), followed by Dermatophyte (D-2).
Key words: Aspergillus, AgNPs, A. terreus, A. nidulans, Malassezia, Trichuris trichiura
How to cite this article: Noor A, Bukhari NT, Kholiskhon R, Alimova Z, Ruzieva M, Saodat Y, Shireen F. Mycosynthesis, Characterization and Antifungal Activity of Silver Nanoparticles Using Aspergillus Species Isolated from Soil. Int J Drug Deliv Technol. 2026;16(12s): 694-701. DOI: 10.25258/ijddt.16.12s.82
Source of support: Nil.
Conflict of interest: None