1*Department of Zoology, RFNS Senior Science College, Akkalkuwa, Maharashtra, India
2Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Maharashtra, India
Adhatoda vasica Nees is a thoroughly established medicinal species with a long history of use in traditional medical systems, however, foliage is the most studied part of the plant and root material is relatively poorly studied. The current study was thus conducted to critically determine the phytochemical profile and develop chromatographic fingerprint of extracts prepared using A. vasica root extract and thus make the process of standardization and quality control feasible. Roots were used to undergo successive solvent extraction with successively polar solvents. The obtained extracts were then subjected to preliminary phytochemical screening and analysis of total phenolic and flavonoid was carried out. The yields of chromatographic profiling came through the use of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) to produce characteristic prints of the fingers. The qualitative and quantitative analysis of alkaloids vasicine and vasicinone that are markers of the drug was performed using HPTLC densitometric analysis. Findings highlighted the strong effect of solvent polarity on the extraction activities and phytochemical patterns with polar extracts having higher levels of phenolics, flavonoids and alkaloid. TLC and HPTLC fingerprints showed different and reproducible patterns, hence proving the existence of various bioactive constituents. Determination of vasicine, vasicinone was further evidence of the appropriateness of roots as a promising source of therapeutic phytochemicals. Together, the research provides a comprehensive phytochemical description and provides standard chromatographic fingerprints of A. vasica roots, providing justification of their possible use in the development of herbal drugs and in quality control.
Keywords: Adhatoda vasica roots; Phytochemical standardization; TLC–HPTLC fingerprinting; Vasicine and vasicinone; Herbal quality control.
How to cite this article: Shaikh MZ, Ransing RD, Usman MRM. Phytochemical Fingerprinting and Quantitative Marker Profiling of Successive Solvent Extracts of Adhatoda vasica Roots Using TLC and HPTLC. Int J Drug Deliv Technol. 2026;16(15s): 966-973. DOI: 10.25258/ijddt.16.15s.108
Source of support: Nil.
Conflict of interest: None