1*Department of Pharmacognosy, Smt. Sharadchandrika Suresh Patil College of Pharmacy, Chopda, Maharashtra, India
Oxidative stress is a critical factor in the pathogenesis of a number of chronic and neurodegenerative diseases and as a result, more attention is given to plant-based antioxidants as safer therapeutic options. This paper sought to assess the antioxidant activity of sequential solvent fractions of stem Tinospora cordifolia using phytochemical profiling and in vitro antioxidant studies. Sequential solvent extraction of the dried stem material was done with increasing polarities of solvents and the extracts were tested in terms of percentage yield, qualitative phytochemical composition, total phenolic content (TPC), total flavonoid content (TFC) as well as antioxidant activity by means of DPPH, ABTS and FRAP.
The findings indicated a polarity-based increment in the extraction yield with the highest being the ethanolic extract. The qualitative phytochemical screening revealed the presence of more phenolics, flavonoids, tannin and glycosides in the medium to high polar extracts. Quantitative analysis has proved that ethanolic, n-butanol and ethyl acetate extracts had high TPC and TFC values. Such extracts were also found to be better antioxidants as indicated by lower IC50s in radical scavenging tests as well as reduced reducing power. According to integrated phytochemical and antioxidant profiles, the ethyl acetate, n-butanol, and ethanolic extracts were found to be lead antioxidant extracts, with the ethanolic being the most active.
Keywords: Tinospora cordifolia, antioxidant activity, phenolic content, flavonoid content, successive solvent extraction.
How to cite this article: Usman MRM, Vadnere GP, Prakash WN. Evaluation of Phytochemical Constituents and Antioxidant Activity in Sequential Solvent Fractions of Tinospora cordifolia Stems. Int J Drug Deliv Technol. 2026;16(15s): 1054-1060. DOI: 10.25258/ijddt.16.15s.116
Source of support: Nil.
Conflict of interest: None