Antiseptic Effect Test of GinLemClo (Ginger, Lemongrass, and Clove) Herbal Mouthwash Against Halitosis-Causing Bacteria in Diabetes Mellitus Mice (In Vivo Method).

Masriadi^1*, Sukmawati², Hasta Handayani Idrus³, Tuti Alawiyah⁴, Mirna Febriani⁴

¹Department of Epidemiology, Faculty of Public Health, Universitas Muslim Indonesia, Makassar, Indonesia
²Department of Pharmacy, Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar, Indonesia
³Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Jakarta, Indonesia
⁴Departement of Dental Material, Faculty of Dentistry, Prof. Dr. Moestopo Beragama University, Jakarta, Indonesia

ABSTRACT

GinLemClo herbal mouthwash made with ginger, lemongrass, and cloves is widely known for its healthful properties. It has been empirically used widely as a treatment for bad breath in people with diabetes mellitus. Therefore, this study aims to test the effect of the antiseptic herbal mouthwash GinLemClo on halitosis-causing bacteria and to reduce halitosis levels in diabetic mice in vivo. This is a true-experimental study conducted with a pretest-posttest-only group design at the Laboratory of Pharmacy and Microbiology, Faculty of Medicine, Hasanuddin University. The number of experimental animals used was 15 male mice suffering from DM and experiencing halitosis. The samples were selected using Random Sampling, while data were analyzed with the paired sample test and one-way ANOVA. The toxicity test conducted by using the GinLemClo herbal mouthwash showed that all mice were in good health. Before treatment, the mice had several bacteria, both pathogenic and non-pathogenic, but after the treatment, only normal flora was found. The treatment also suppressed the growth of halitosis-causing bacteria in DM mice (p 0.000) and reduced halitosis levels with f-count (18,300) > f table (3.74). Based on the results, GinLemClo herbal mouthwash can be used as an antiseptic to inhibit the cause, and reduce levels of halitosis

Keywords: Antibacterial, GinLemClo, Herbal Mouthwash, Diabetes Mellitus

How to cite this article:Masriadi, Sukmawati, Idrus HH, Alawiyah T, Febriani M, Antiseptic Effect Test of GinLemClo (Ginger, Lemongrass, and Clove) Herbal Mouthwash Against Halitosis-Causing Bacteria in Diabetes Mellitus Mice (In Vivo Method). .Int J Drug Deliv Technol. 2026;16(1s): 1102-1110 DOI: 10.25258/ijddt.16. 1102-1110