Objectives: The study was undertaken to develop and validate a reliable HPLC method for the quantitative estimation of the flavonoid quercetin in different extracts of Terminalia arjuna, with an emphasis on supporting quality evaluation and standardization of herbal formulations. Methods: The raw drug was procured, processed, and subjected to extraction to obtain aqueous, ethanolic, and milk-based decoction (Arjuna Ksheer Paka) extracts. Preliminary phytochemical screening was performed to identify major classes of phytoconstituents. Total phenolic and flavonoid contents were estimated using standard colorimetric methods, while antioxidant activity was assessed by IC₅₀ determination (DPPH assay). HPLC method development was carried out using a C18 column with a mobile phase consisting of 0.1% formic acid and acetonitrile (60:40 v/v), followed by method validation. Findings: Phytochemical analysis confirmed the presence of flavonoids, polyphenols, cardiac glycosides, and related constituents. Total phenolic content was found to be 142.46 ± 0.50, 482.47 ± 1.52, and 58.20 ± 5.77 mg GAE/100 g for aqueous, ethanolic, and milk-based extracts respectively, while total flavonoid content was 179.21 ± 0.62, 89.81 ± 7.23, and 51.23 ± 0.77 mg QE/g. Antioxidant activity carried out by DPPH assay showed IC₅₀ values of 33.77 ± 0.43, 17.19 ± 1.50, and 31.40 ± 1.95 µg/ml for the respective extracts. The validated HPLC method enabled accurate quantification of quercetin, which was found to be 2.10 ± 1.17 mg per 400 mg of extract. Novelty: The novelty of this study lies in the integration of a validated HPLC method for quercetin estimation with comparative phytochemical and antioxidant profiling of aqueous, alcoholic, and milk-based Terminalia arjuna extracts. Inclusion of the traditional Arjuna Ksheer Paka formulation offers a unique insight into classical preparations..
Keywords: Quercetin, HPLC, T.Arjuna, standardisation, Arjuna Ksheer Paka..
How to cite this article: Gandhi R, Salunkhe R, Lehri A, Patil S.; Standardizing Arjuna Extracts: HPLC, Phytochemical, and Antioxidant Analysis for Consistent Quality..Int J Drug Deliv Technol. 2026;16(1s): 596-604; DOI: 10.25258/ijddt.16. 596-604