Background:Biopolymeric scaffolds play a critical role in tissue engineering by providing structural support and promoting cellular responses. Although polyvinyl alcohol (PVA) offers mechanical stability and collagen enhances biocompatibility, their combination lacks inherent biological activity. Incorporation of plant-based bioactive compounds may enhance the therapeutic functionality of such scaffolds. Xanthium strumarium is a medicinal plant known for its anti-inflammatory and osteogenic properties. Aim:To fabricate and evaluate a PVA/Collagen bioscaffold enriched with Xanthium strumarium leaf extract for its anti-inflammatory and osteogenic differentiation potential. Materials and Methods: A composite bioscaffold was fabricated using 10% PVA and 1% collagen, incorporating methanolic Xanthium strumarium leaf extract through a freeze-drying technique. Anti-inflammatory activity was assessed using LPS-stimulated RAW 264.7 macrophages by measuring TNF-α and IL-6 expression through ELISA and quantitative real-time PCR. Osteogenic differentiation was evaluated using MG-63 cells cultured on the scaffold for 14 days, followed by Alizarin Red S staining and quantitative mineralization analysis. Surface morphology and elemental composition were analyzed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDAX). Results:The extract-enriched scaffold significantly reduced TNF-α and IL-6 expression in LPS-stimulated macrophages, demonstrating notable anti-inflammatory activity comparable to standard anti-inflammatory control. MG-63 cells exhibited enhanced mineralized matrix formation, confirmed by positive Alizarin Red S staining and increased calcium deposition. SEM analysis revealed a highly porous and interconnected scaffold architecture, while EDAX confirmed the presence of bioactive elemental constituents. Conclusion:The PVA/Collagen bioscaffold incorporated with Xanthium strumarium leaf extract exhibited significant anti-inflammatory and osteogenic differentiation potential in vitro. The integration of plant-derived bioactive compounds imparted therapeutic functionality to the scaffold, highlighting its potential application in wound healing and bone tissue engineering. Further in vivo investigations are warranted to validate its clinical relevance..
Keywords: Anti-inflammatory, osteogenecity , Xanthium strumarium, PVA / Collagen Bioscaffold, Wound healing
How to cite this article:Kiran KD, Sundari SKK, Sreenivasagan S; Anti-Inflammatory Activity Of Pva/Collagen Bioscaffold Enriched With Xanthium Strumarum Leaf Extract..Int J Drug Deliv Technol. 2026;16(1s): 706-709; DOI: 10.25258/ijddt.16. 706-709