Background: Antimicrobial resistance (AMR) represents one of the most pressing global health challenges of the 21st century, with an estimated 1.27 million deaths directly attributable to bacterial antimicrobial resistance in 2019. Antimicrobial stewardship programs (ASPs) have emerged as critical interventions to optimize antimicrobial use, reduce resistance, and improve patient outcomes. While traditionally focused on physicians and clinical pharmacists, contemporary ASPs increasingly recognize the vital contributions of multidisciplinary healthcare teams, including nursing specialists, pharmacy technicians, dental technologists, health information technicians, medical informatics technicians, radiology technicians, and health assistant nurses. Objective: This comprehensive review examines the evidence for multidisciplinary involvement in antimicrobial stewardship, evaluates the specific roles and contributions of various healthcare professionals, assesses knowledge, attitudes, and practices toward AMR and infection prevention, and identifies barriers and facilitators to effective implementation. Methods: A narrative review was conducted based on current literature (2023-2025) focusing on multidisciplinary antimicrobial stewardship programs, healthcare workers' knowledge and practices regarding AMR, infection prevention and control education, and outcomes of team-based interventions. Evidence from systematic reviews, meta-analyses, cohort studies, and quality improvement initiatives was synthesized. Results: Global meta-analyses reveal that healthcare workers demonstrate moderate knowledge of AMR (56.5% with good knowledge), positive attitudes (74%), but suboptimal preventive practices (44%). Multidisciplinary ASP interventions consistently demonstrate significant improvements in antibiotic utilization (reductions of 15-48% in defined daily doses per 100 patient-days), improved guideline adherence (from 47% to 72.5%). Pharmacy technicians, nursing staff, laboratory personnel, and allied health professionals play increasingly recognized roles in medication verification, patient education, specimen collection, diagnostic stewardship, and infection control implementation. Conclusion: Effective antimicrobial stewardship requires integration of the entire healthcare team, with clearly defined roles, structured training programs, interprofessional collaboration, and institutional support. Targeted educational interventions must address the knowledge-practice gap, particularly in resource-constrained settings. Future ASPs should formalize contributions from all healthcare disciplines, implement competency-based training frameworks, and foster a culture of collective accountability in combating antimicrobial resistance
Keywords: Antimicrobial stewardship, multidisciplinary team, antimicrobial resistance, infection prevention and control, healthcare workers, pharmacy technicians, nursing specialists, allied health professionals, patient safety, quality improvement
How to cite this article:Alsharif FSS, Safer SM, Almutari AH, Al Shaibani SAA, Alotaibi TA, Bin Dous MS, MoPrince RAM, Alsuliman NK, Alsuliman GK, A Comprehensive Overview of How Multidisciplinary Healthcare Teams Contribute to Antimicrobial Stewardship Programs. 2026;16(1s): 778-790; DOI: 10.25258/ijddt.16. 778-790