1Department of Biochemistry, The Oxford College of Science, Arts, Commerce and Management, No. 32, 17th B Cross, Sector-IV, HSR Layout, Bengaluru – 560102, Karnataka, India
2BioNome, No.17, Temple Road, Hennur Cross, Bangalore-560043, Karnataka, India
*Corresponding Author: Dr. Sangita Roy, Professor and Head, Department of Biochemistry, The Oxford College of Science, Arts, Commerce and Management, Bengaluru, Karnataka, India
Received: 16th Dec, 2025; Revised: 8th Feb 2026; Accepted: 12th Feb, 2026; Available Online: 28th Feb, 2026
Silver nanoparticles (AgNPs) have been extensively explored due to their varied biological properties. Annona muricata. L leaf extract-derived silver nanoparticles (AM-AgNPs) were synthesized and characterized using UV–Visible spectroscopy, ATR-FTIR spectroscopy, and Scanning Electron Microscopy (SEM). A characteristic surface plasmon resonance peak at 440 nm was detected in the UV–Visible absorption spectrum, confirming AgNPs formation. SEM analysis revealed particle sizes ranging from 350 to 650 nm, while ATR-FTIR identified functional groups involved in the reduction and capping of silver ions, providing insights into surface morphology and stabilization. Phytochemical profiling by LC–MS and DPPH study revealed a 25% enhancement in antioxidant activity in the AM-AgNPs compared to A. muricata leaf (AML) extract alone. In addition, a sustained release of 77.3% over 24 hr. established effective entrapment and prolonged delivery potential. The entrapment efficiency of phytochemicals within AM-AgNPs was found to be in the range of 74.23–81.09%, representing strong phytochemical association with the nanoparticles. Cytotoxicity evaluated by MTT assay using MCF-7 breast cancer cell lines showed concentration-dependent growth inhibition, with an IC₅₀ value of 74.51 µg/mL.
Keywords: Antioxidant, Anti Proliferative, Silver nanoparticles, MCF-7, MTT Assay
How to cite this article: R Santhosh P, Reddy V, N Sonu, C Chandan, KS Bhanu Prakash, Sharma S, Roy S, Green synthesis of Silver Nanoparticles from Annona muricata L. Leaf Extract: Characterization and Assessment of Antioxidant and Anti-Cancer Potential. Int J Drug Deliv Technol. 2026; 16(2): 464-474; DOI: 10.25258/ijddt.16.2.52
Source of support: Nil.
Conflict of interest: None